利用鸡F<SUB>2</SUB>资源群体构建1号染色体遗传连锁图谱

柳晓峰，王守志，胡晓湘，高宇，王启贵，张慧，李宁，李辉

doi:10.1360/yc-007-0977

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2007 , Vol. 29 >Issue 8: 977 - 977―981

DOI: https://doi.org/10.1360/yc-007-0977

研究报告

利用鸡F₂资源群体构建1号染色体遗传连锁图谱

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1. 东北农业大学动物科技学院, 哈尔滨150030;
2. 中国农业大学农业生物技术国家重点实验室, 北京100094

收稿日期: 2006-12-07

修回日期: 2007-02-07

网络出版日期: 2007-08-10

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A genetic linkage map of chicken chromosome 1 in an F₂ resource population

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1. College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, China;
2. National Laboratories for Agribiotechnology, China Agricultural University, Beijing 100094, China

Received date: 2006-12-07

Revised date: 2007-02-07

Online published: 2007-08-10

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摘要

在鸡1号染色体上选取23个微卫星标记，利用东北农业大学鸡F2资源群体构建了遗传连锁图谱。选用369只F2个体用于基因型测定。结果表明23个微卫星位点除MCW0058为低度多态，其他位点均为中高度多态。构建的连锁图谱覆盖1号染色体全长，总共637.9 cM。MCW0115和ROS0025标记顺序与EL图谱不同，但与WAU图谱一致。其他标记顺序与3大参考家系标记顺序一致，图谱总长和标记间距离大于3大参考家系。此连锁图谱的构建为数量性状位点（QTL）定位奠定了良好的基础。

关键词： 鸡; F2资源群体; 微卫星; 连锁图谱

本文引用格式

柳晓峰，王守志，胡晓湘，高宇，王启贵，张慧，李宁，李辉 . 利用鸡F₂资源群体构建1号染色体遗传连锁图谱[J]. 遗传, 2007 , 29(8) : 977 -977―981 . DOI: 10.1360/yc-007-0977

Abstract

Three hundred and sixty-nine F2 individuals produced from Northeast Agricultural University Resource Population (NEAURP) were genotyped by 23 fluorescent microsatellite markers on chromosome 1. The characterization of these mi-crosatellites was moderate or high polymorphic except marker MCW0058 which was low polymorphic. The length of the sex averaged linkage map was 637.9 cM. The order of MCW0115 and ROS0025 disagreed with that of EL map, but con-sisted with that of W map. The intervals of markers were larger than those of three reference families.

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