综述

RNA扩增的研究进展

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  • 1.中国农业科学院生物技术研究所, 北京 100081;
    2.首都师范大学生命科学学院, 北京 100037

收稿日期: 2007-01-08

  修回日期: 2007-02-26

  网络出版日期: 2007-08-10

Progress of RNA amplification

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  • 1. Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China;
    2. College of Life Science, Capital Normal University, Beijing 100037, China

Received date: 2007-01-08

  Revised date: 2007-02-26

  Online published: 2007-08-10

摘要

RNA扩增是伴随着基因芯片技术的应用而发展起来的一项技术,但不仅仅应用于芯片杂交。本文对RNA扩增的技术方法进行了综述,重点介绍了mRNA的线性扩增、指数扩增以及两者相结合的扩增方法以及优缺点,对microRNA的扩增方法也作了介绍,并对将来的发展作了展望。

本文引用格式

李轶女,胡英考,张志芳,沈桂芳 . RNA扩增的研究进展[J]. 遗传, 2007 , 29(8) : 907 -914 . DOI: 10.1360/yc-007-0907

Abstract

The technology of RNA amplification is developing with the application of gene chip and it is not only adapted for chip hybridization, but also applied in other purposes as well. The methodologies of RNA amplification were reviewed, focused on linear, exponential and linear plus exponential amplifications. The amplification of microRNA was also introduced and a prospect of RNA amplification was made.

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