聚丙烯酰胺凝胶快速、高效银染方法的建立

梁宏伟，王长忠，李忠，罗相忠，邹桂伟

doi:10.3724/SP.J.1005.2008.01379

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2008 , Vol. 30 >Issue 10: 1379 - 1382

DOI: https://doi.org/10.3724/SP.J.1005.2008.01379

技术与方法

聚丙烯酰胺凝胶快速、高效银染方法的建立

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1. 中国水产科学研究院长江水产研究所,农业部淡水鱼类种质资源与生物技术重点开放实验室, 荆州 434000;
2. 中国水产科学研究院淡水渔业研究中心, 无锡 214081;
3. 华中农业大学水产学院, 武汉 430070

收稿日期: 2008-03-28

修回日期: 2008-08-01

网络出版日期: 2008-10-10

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Improvement of the silver-stained technique of polyacrylamide gel electrophoresis

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1. Key Laboratory of Fish Germplasm Resources and Biotechnology Certificated by Ministry of Agriculture,Yangtze River Fisheries Re-search Institute, Chinese Academy of Fishery Sciences, Jingzhou 434000, China;
2. Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China;
3. College of Fisheries, Huazhong Agricultural University, Wuhan 430070, China

Received date: 2008-03-28

Revised date: 2008-08-01

Online published: 2008-10-10

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摘要

摘要: 聚丙烯酰胺凝胶电泳目前已经广泛应用在SSR标记、SNP标记以及遗传图谱的构建等过程中, 但是一直以来凝胶银染方法由于染色时间长、染色步骤繁琐, 使得对于开展大批量的实验研究极为不利。文章通过对常规方法的改良, 建立了一个银染步骤只需要10 min, 整个染胶过程只需约20 min的快速、高效的银染方法。

关键词： 聚丙烯酰胺凝胶; DNA; 银染方法

本文引用格式

梁宏伟，王长忠，李忠，罗相忠，邹桂伟 . 聚丙烯酰胺凝胶快速、高效银染方法的建立[J]. 遗传, 2008 , 30(10) : 1379 -1382 . DOI: 10.3724/SP.J.1005.2008.01379

Abstract

Abstract: Polyacrylamide gel electrophoresis is widely applied in the SSR, SNP markers analysis and the construction of genetic map. It is disadvantage to use conventional silver-stained methods in experiment due to taking a very long time and having fussy steps. In this paper, a silver-stained method of DNA in PAGE gel was improved. The improved method is rapid banding which only takes about 20 min, and the high definition picture was obtained.

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