摘要: 采用PCR-SSCP技术和DNA测序的方法, 对比利时兔、天府黑兔、齐卡巨型兔、哈尔滨白兔以及加利福尼亚兔5个肉兔品种的生长激素受体(Growth hormone receptor, GHR)基因进行单核苷酸多态性分析。结果发现了2个单核苷酸多态位点, 分别位于外显子10的705(T→C)和810 (C→T)。通过最小二乘分析SNPs及其与屠宰性状的关系发现, 基因型AA和MM所对应的活重、全净膛、屠宰率最小二乘均值都显著低于基因型BB和NN(P<0.05)。但各基因型饲料转化率最小二乘均值差异不显著(P>0.05)。由此可以初步推断, GHR基因是影响兔体重和屠宰率等屠宰性状的主要候选基因。

Abstract: Five rabbit populations (Belgian hare, Tianfu black rabbit, Great line of Zika rabbit, Harbin white rabbit, and Cali-fornia rabbit) were used to analyze the polymorphism of growth hormone receptor (GHR) gene by PCR-SSCP. Results indi-cated that there were two mutation sites (C705T and C810T) in the 5 populations. The least square analyses showed that the live weight, visceraste weight, and slaughter percentage of AA and MM genotypes were significantly lower than BB and NN genotypes (P<0.05). In contrast, the GHR polymorphism had no significant difference for least squares means of feed trans-formation efficiency (P>0.05). It suggested that GHR gene may be a candidate gene responsible for butcher trait in rabbit.