研究报告

郏县红牛DGAT2基因多态性与生长性状的相关性分析

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  • 1. 西北农林科技大学动物科技学院 陕西省农业分子生物学重点实验室, 杨凌 712100;
    2. 徐州师范大学细胞与分子生物学研究所, 徐州 221116;
    3. 山西师范大学生命科学院动科系, 临汾 041000;
    4. 河南省肉牛工程技术研究中心, 郑州 450003

收稿日期: 2007-08-16

  修回日期: 2007-11-15

  网络出版日期: 2008-03-10

Association of polymorphisms of DGAT2 gene with growth traits in Jiaxian red cattle

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  • 1. College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling 712100, China; 2. Institute of Cellular and Molecular Biology, Xuzhou Normal University, Xuzhou 221116, China; 3. College of Life Science, Shanxi Normal University, Linfen 041000, China;
    4. Research Center of Cattle Engineering Technology in Henan, Zhengzhou 450003, China

Received date: 2007-08-16

  Revised date: 2007-11-15

  Online published: 2008-03-10

摘要

利用PCR-RFLP技术对随机抽取的92头郏县红牛的DGAT2基因进行多态性分析。结果表明, DGAT2基因第6内含子的PCR扩增产物被限制性酶TaqⅠ酶切消化后表现多态性。经电泳检测后, 显出AA、AB两种不同基因型。该基因座处于Hardy-Weinberg平衡状态, 通过对郏县红牛DGAT2基因多态性与体重及体尺指标的方差分析显示:郏县红牛DGAT2基因不同基因型对郏县红牛体重和体长有显著影响(P<0.05), 而对其他体尺指标在统计学上无显著影响(P>0.05), 且初步认为AB基因型对选择有正向效应。

本文引用格式

毛海霞,陈宏,陈付英,张春雷,王新庄,王居强 . 郏县红牛DGAT2基因多态性与生长性状的相关性分析[J]. 遗传, 2008 , 30(3) : 329 -332 . DOI: 10.3724/SP.J.1005.2008.00329

Abstract

The polymorphisms of DGAT2 gene in 92 Jiaxian red cattle were analyzed using PCR-RFLP method. Results showed that the PCR products digested with TaqI demonstrated polymorphisms, and showed two kinds of genotype: AA and AB. This polymorphic locus of DGAT2 gene was at Hardy-Weinberg equilibrium (P>0.05). The results of general linear model analysis showed that polymorphisms of DGAT2 gene had significant association (P<0.05) with body weight and body length, while had no significant association with others growth traits (P>0.05). Therefore, genotype AB may have positive effect on body weight.

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