采用多重PCR的方法, 对其反应条件进行优化, 以获得用于丝羽乌骨鸡遗传分析的稳定PCR体系。18个微卫星标记共获得5个扩增效果理想的组合, 其中3个为四重PCR, 2个为三重PCR。将扩增产物进一步合并为3组, 用ABI 3100-Avant遗传分析仪检测。实验结果表明, 所选用的微卫星标记表现出丰富的多态性, 可成功地应用于丝羽乌骨鸡的遗传分析。

In order to obtain the stable PCR combinations for silkies genetic analysis, multiplex PCR was used and its reaction condition was optimized. Five combinations of multiplex PCR with good effects were obtained from eighteen microsatellite markers. Three of them were quadruplex-PCRs, and two were triplex-PCRs. The products of multiplex PCR were further combined into three sets for the electrophoresis on ABI 3100-Avant Genetic Analyzer. The results suggested that the eighteen microsatellite markers could be successfully applied to the silkies genetic analysis.