首次利用SMARTTM技术构建了中国普通野生稻中最原始类型——元江普通野生稻生长旺盛时期叶片的cDNA文库。该cDNA文库未扩增和扩增后的滴度分别为1.1×10⁶ pfu/mL和3.98×10⁷ pfu/mL, 重组率为91%, 插入片段大小为500～2 000 bp。测定的部分cDNA序列进行BLAST比较, 发现这些cDNA片段与日本晴栽培稻同源性很高, 达到98%以上。本研究为进一步分析这些cDNA片段的结构、功能和探讨元江普通野生稻在栽培稻演化中的地位奠定了基础。

The cDNA library of Yuanjiang Oryza rifupongon leaf was constructed by using SMARTTM technology. The titers of the non-amplified library and the amplified library were 1.1×10⁶ pfu/mL and 3.98×10⁷ pfu/mL, respectively. The recombi-nation rate was more than 91%. The DNA sequence length of the most cDNAs in the library was between 500-2 000 bp. Some cDNAs chosen by random were sequenced. After BLAST analysis of some cDNAs, their possible function were pre-dicted. It is found that these cDNAs show 98% similarity to Oryza sativa japonica in the NCBI database. These provided a base for further study on the structure and function of these cDNAs and evolutionary process of Yuanjiang Oryza rifupongon .