微卫星在种公牛个体识别与亲缘鉴定方面的应用

王静，刘丑生，张利平，王志刚，于福清，张桂香，孙秀柱，韩旭，魏玉春

doi:10.3724/SP.J.1005.2009.00285

遗传 >

2009 , Vol. 31 >Issue 3: 285 - 295

DOI: https://doi.org/10.3724/SP.J.1005.2009.00285

研究报告

微卫星在种公牛个体识别与亲缘鉴定方面的应用

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1. 兰州职业技术学院, 兰州 730070;
2. 农业部全国畜牧总站畜禽牧草种质资源保存利用中心, 北京100193;
3. 甘肃农业大学动物科学技术学院, 兰州730070;
4. 西北农林科技大学动物科技学院, 杨凌 712100

收稿日期: 2008-03-28

修回日期: 2008-08-23

网络出版日期: 2009-03-10

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Individual identification and paternity testing of bulls using microsatellite

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1. Lanzhou Vocational Technology College, Lanzhou 730070, China;
2. National Center of Preservation and Utilization of Genetic Resources of Domestic Animals and Forage, National Animal Husbandry and Veterinary Service, Beijing 100193, China;
3. College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, China;
4. College of Animal Science and Technology, Northwest Sci-Tech University of Agriculture & Forestry, Yangling 712100, China

Received date: 2008-03-28

Revised date: 2008-08-23

Online published: 2009-03-10

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摘要

采用美国ABI公司牛亲子鉴定试剂盒(Bovine Paternity PCR Typing Kit, 包括11个常染色体)和3个自选的Y染色体微卫星座位, 检测我国部分种公牛站肉用种公牛14个微卫星座位的多态性分布, 评估其遗传多样性, 并探讨其用于个体识别与亲缘鉴定的可行性。结果表明: 种公牛在14个微卫星座位中遗传多样性均较高, 其中MCM158座位的平均多态信息含量最高达到0.888, ETH10座位的最低, 为0.482。单个座位的个体识别能力在0.715~0.968之间, 累积个体识别能力为99.99%, 累计非父排除率达到99.99%, 表明采用的14个位点适用于个体识别和亲缘鉴定。

关键词： 种公牛; 微卫星; 个体识别; 亲缘鉴定

本文引用格式

王静，刘丑生，张利平，王志刚，于福清，张桂香，孙秀柱，韩旭，魏玉春 . 微卫星在种公牛个体识别与亲缘鉴定方面的应用[J]. 遗传, 2009 , 31(3) : 285 -295 . DOI: 10.3724/SP.J.1005.2009.00285

Abstract

The polymorphism distributions of 14 microsatellite loci were detected using the Bovine Paternity PCR Typing Kit (including11 X-STR) and 3 selected Y-STR microsatellite DNA markers. The genetic diversity were evaluated, and the feasibility of the application to individual identification and paternity testing were discussed. The results showed that all the 14 microsatellite loci had genetic polymorphisms in bulls, and the polymorphism information content (PIC) in loci MCM158 was the biggest (0.888), while the ETH10 was the lowest (0.482). Power of discrimination (DP) value of the 14 STR loci ranged from 0.715 to 0.968. The Cumulate DP (CDP) was 99.99%, and the Cumulate PE (CPE) also reached 99.99%. These results indicate that the 14 microsatellites can be applied to the individual identification.
Keywords: bull; microsatellite;individual identification; paternity testing

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