DArT技术的原理及其在植物遗传研究中的应用

洪义欢，肖宁，张超，苏琰，陈建民

doi:10.3724/SP.J.1005.2009.00359

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2009 , Vol. 31 >Issue 4: 359 - 364

DOI: https://doi.org/10.3724/SP.J.1005.2009.00359

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DArT技术的原理及其在植物遗传研究中的应用

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1. 扬州大学生物科学与技术学院, 扬州 225009;
2. 江苏里下河地区农业科学研究所, 扬州 225000

收稿日期: 2008-10-04

修回日期: 2008-11-07

网络出版日期: 2009-04-10

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Principle of diversity arrays technology (DArT) and its applications in genetic research of plants

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1. College of Bioscience and Biotechnology, Yangzhou University, Yangzhou 225009, China;
2. Agricultural Scicece Institute of Jiangsu Lixiahe District, Yangzhou 225000, China

Received date: 2008-10-04

Revised date: 2008-11-07

Online published: 2009-04-10

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摘要

DArT是一种依赖于基因芯片杂交基础之上的分子标记技术, 由于其具有省时、高通量、低花费、无需预知研究对象的DNA序列等优点而被应用到植物遗传研究中。文章介绍了DArT技术的原理、特点、具体操作方法及其目前在植物遗传研究中的应用。

关键词： 杂交; DArT技术; 分子标记

本文引用格式

洪义欢，肖宁，张超，苏琰，陈建民 . DArT技术的原理及其在植物遗传研究中的应用[J]. 遗传, 2009 , 31(4) : 359 -364 . DOI: 10.3724/SP.J.1005.2009.00359

Abstract

Diversity arrays technology (DArT) is a microarray hybridization-based molecular marker technique, and a novel method to discover genetic polymorphic markers. DArT has recently been applied in genetic research of plants be-cause of its characteristics of sequence-independent, high–throughput, fast, low-cost. We briefly introduce the principle, characteristics, protocol of DArT and its application in genetic research of plants.

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