藏鸡诱导型一氧化氮合酶基因低氧适应功能分析

张浩，强巴央宗，赵春江，鲍海港，凌遥，吴常信

doi:10.3724/SP.J.1005.2009.00400

遗传 >

2009 , Vol. 31 >Issue 4: 400 - 406

DOI: https://doi.org/10.3724/SP.J.1005.2009.00400

研究报告

藏鸡诱导型一氧化氮合酶基因低氧适应功能分析

展开

1. 中国农业大学动物科技学院, 北京100193;
2. 西藏农牧学院动物科学系, 西藏林芝 860000

收稿日期: 2008-09-25

修回日期: 2008-11-13

网络出版日期: 2009-04-10

收起

Function of inducible nitric oxide synthase on adaptability to hypoxia in Tibetan chicken

Expand

1. College of Animal Science and Technology, China Agricultural University, Bejing 100193, China;
2. College of Agriculture and Animal Husbandry, Tibet University, Linzhi 860000, China

Received date: 2008-09-25

Revised date: 2008-11-13

Online published: 2009-04-10

Fold

摘要

低氧刺激诱导型一氧化氮合酶(Inducible nitric-oxide synthase, iNOS)催化产生NO, 增加血流, 改善组织供氧。文章采用测序和PCR-RFLP技术检测藏鸡及低地鸡iNOS基因编码区、5′侧翼区(2.0 kb片段)序列和3′侧翼序列SNP, 并测定低氧和常氧孵化时鸡胚尿囊绒毛膜组织iNOS基因表达量和酶活力。结果在iNOS基因 5′侧翼区发现一个与低氧适应相关的藏鸡高频率突变SNP位点(-870C→T), 藏鸡该突变的等位基因T频率高于低地鸡种。藏鸡iNOS基因表达量和酶活力在低氧孵化环境中多高于矮小鸡。结果表明藏鸡群体iNOS基因的突变及其低氧表达量的增加是其适应低氧环境的重要基础。

关键词： 基因表达; SNP; iNOS基因; 低氧适应; 藏鸡

本文引用格式

张浩，强巴央宗，赵春江，鲍海港，凌遥，吴常信 . 藏鸡诱导型一氧化氮合酶基因低氧适应功能分析[J]. 遗传, 2009 , 31(4) : 400 -406 . DOI: 10.3724/SP.J.1005.2009.00400

Abstract

The inducible nitric-oxide synthase (iNOS) can be induced by hypoxia to produce NO, which regulates blood flow and improves oxygen delivery to tissues. In present study, SNPs in coding and 5′-flanking regions of iNOS gene were examined in Tibetan chicken and lowland chicken using sequence and PCR-RFLP methods, and the quantitive express of mRNA of iNOS and the enzyme activity were measured in chorioallantoic membrane of chick embryo that was incubated under hypoxic and normal conditions. The results showed that a special SNP (-870C→T)was found in 5′-flanking region of iNOS gene. Tibetan chicken has higher frequencies in mutation allele T than lowland chicken. In hypoxic incubation, the mRNA expression of iNOS and enzyme activity were higher in Tibetan chicken than that in Dwarf chicken. It was con-cluded that the mutation of iNOS and hypoxic increase of its expression were important base for adaptability to hypoxia in Tibetan chicken.

Options

文章导航

〈

〉

www.chinagene.cn
备案号：京ICP备09063187号-4
总访问:,今日访问:,当前在线:

模态框（Modal）标题

摘要

本文引用格式

Abstract