免疫荧光染色检测克隆牛X染色体组蛋白H3 Lys 4 二甲基化修饰

曹更生，张洪，周文平，李宁

doi:10.3724/SP.J.1005.2009.00611

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2009 , Vol. 31 >Issue 6: 611 - 614

DOI: https://doi.org/10.3724/SP.J.1005.2009.00611

研究报告

免疫荧光染色检测克隆牛X染色体组蛋白H3 Lys 4 二甲基化修饰

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1. 河南大学生命科学学院, 开封 475004;
2. 中国农业大学农业生物技术国家重点实验室, 北京 100193

收稿日期: 2008-10-10

修回日期: 2009-03-12

网络出版日期: 2009-06-10

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Immunofluorescence patterns of histone H3 lysine 4 dimethylation on X chromosomes of different cloned cattle

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1. College of Life Science, Henan University, Kaifeng 475004, China;
2. State Key Laboratories of Agrobiotechnology, China Agricultural University, Beijing 100193, China

Received date: 2008-10-10

Revised date: 2009-03-12

Online published: 2009-06-10

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摘要

利用免疫荧光染色, 检测胎儿成纤维细胞(FFB)和输卵管上皮细胞(FOV)来源的克隆牛X中期染色体组蛋白H3 K4m2修饰状况。结果发现, 这两种细胞系来源的克隆牛耳组织细胞系X染色体H3 K4m2修饰与供体核FFB细胞系和常规繁殖牛耳组织细胞系的修饰基本一致, 而与供体核FOV细胞系有较大差异。

关键词： 克隆牛; 表观遗传修饰; H3 K4m2; X染色体; 免疫荧光染色

本文引用格式

曹更生，张洪，周文平，李宁 . 免疫荧光染色检测克隆牛X染色体组蛋白H3 Lys 4 二甲基化修饰[J]. 遗传, 2009 , 31(6) : 611 -614 . DOI: 10.3724/SP.J.1005.2009.00611

Abstract

Using immunofluorescence staining, the patterns of histone H3 K4m2 of metaphase X chromosomes were measured in cloned cattle derived from FFB and FOV donor cells. The results demonstrated that the modification pattern of H3 K4m2 in the ear tissue of all clones was largely consistent with that of the donor cell line FFB and conventionally pro-duced cattle, but different from that of the donor cell line FOV.

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