小麦糯性基因的多重PCR分子鉴定

卢龙斗，侯彩玲，陈龙，殷贵鸿，邓传良，高武军，杨绪勤，谭光轩

doi:10.3724/SP.J.1005.2009.00844

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2009 , Vol. 31 >Issue 8: 844 - 848

DOI: https://doi.org/10.3724/SP.J.1005.2009.00844

研究报告

小麦糯性基因的多重PCR分子鉴定

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1. 河南师范大学生命科学学院, 新乡453007;
2. 河南省周口师范学院生命科学系, 周口466000;
3. 河南省周口市农业科学院, 周口466001

收稿日期: 2008-12-23

修回日期: 2009-04-05

网络出版日期: 2009-08-10

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Molecular identification on Waxy genes in wheat using multiple-PCR

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1. College of Life Science, Henan Normal University, Xinxiang 453007, China;
2. Department of Life Science, Zhoukou Normal University, Henan Province Zhoukou 466000, China;
3. Zhoukou Institute of Agricultural Science, Henan Province Zhoukou 466001, China

Received date: 2008-12-23

Revised date: 2009-04-05

Online published: 2009-08-10

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摘要

采用多重 PCR 的方法, 对其反应条件进行优化, 以获得用于小麦糯性(Wx)基因分析的稳定PCR体系。应用两对引物, 分别扩增小麦 Wx-A1、Wx-B1、Wx-D1 基因, 目的片段大小分别为: 230 bp/265 bp、854 bp和 204 bp。经反复验证, 结果准确可靠, 重复性好, 成本低, 可以在同一PCR反应体系中对 3 个Wx 基因进行同时筛选鉴定。该体系可用于 Wx 蛋白基因的分子标记辅助选择, 可以提高小麦淀粉品质评价和糯麦选育的效率。

关键词： 小麦; Wx基因; 多重PCR; 分子标记辅助选择

本文引用格式

卢龙斗，侯彩玲，陈龙，殷贵鸿，邓传良，高武军，杨绪勤，谭光轩 . 小麦糯性基因的多重PCR分子鉴定[J]. 遗传, 2009 , 31(8) : 844 -848 . DOI: 10.3724/SP.J.1005.2009.00844

Abstract

Multiple-PCR was conducted to establish a stable PCR system for identifying the three Wx genes in wheat. Two pairs of primers were employed to amplify Wx-A1, Wx-B1, and Wx-D1 genes of wheat, with the target sequences of 230 bp/265 bp, 854 bp, and 204 bp, respectively. The results showed that Wx-A1, Wx-B1, and Wx-D1 can be detected si-multaneously in a single reaction. This method proved to be repeatable and low cost for evaluation of wheat quality proper-ties in breeding program. This multiple-PCR technique can be efficiently used in marker-assisted selection for Wx genes, which will improve selection procedure for waxy wheat.

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