结合SSR标记和STS标记对家蚕无鳞毛翅基因的定位
收稿日期: 2009-05-26
修回日期: 2009-09-13
网络出版日期: 2010-01-15
基金资助
国家重点基础研究发展计划(973计划)项目(编号：2005CB121004), 农业部行业专项(编号：nyhyzx07-020)和江苏省青蓝工程项目资助
Mapping of non-lepis wing gene nlw in silkworm (Bombyx mori) using SSR and STS markers
Received date: 2009-05-26
Revised date: 2009-09-13
Online published: 2010-01-15
家蚕突变表型无鳞毛翅(non-lepis wing, nlw)由隐性基因nlw控制。由于家蚕雌性不发生交换, 文章采用有鳞毛翅品系P50和无鳞毛翅品系U06两个品系组配F1代及BC1回交群体, (U06×P50)×U06和U06×(U06×P50)分别记作BC1F和BC1M, 根据已经构建的家蚕SSR分子标记连锁图谱及已经发表的有关序列对nlw基因进行了连锁及定位分析。得到8个与nlw基因连锁的SSR(Simple sequence repeat)标记和1个STS(Sequence-tagged sites)标记。BC1F群中的所有正常翅个体均表现出与(U06×P50)F1相同的杂合带型; 而所有无鳞毛个体带型与亲本U06一致, 为纯合型。利用BC1M群体构建了关于nlw基因的遗传连锁图, 连锁图的遗传距离为125.7 cM, 与nlw基因最近的引物为STS标记cash2p, 图距为11.4 cM。
王修业，李木旺，赵云坡，徐安英，郭秋红，黄勇平，郭锡杰 . 结合SSR标记和STS标记对家蚕无鳞毛翅基因的定位[J]. 遗传, 2010 , 32(1) : 54 -58 . DOI: 10.3724/SP.J.1005.2010.00054
The non-lepis wing of silkworm (Bombyx mori) is controlled by the recessive gene, nlw. Owning to lack of crossing over in females, the reciprocal backcrossed F1 (BC1) progenies were used for linkage analysis and mapping of nlw based on the SSR linkage map and STS markers using the wild type (+nlw/+nlw) silkworm strain P50 and U06 with scaleless wing (nlw/nlw). The nlw gene was linked to eight SSR markers and one STS marker. All the individuals with the wild type in the BC1F (Using F1 as female to backcross to the recessive parent, that is (U06×P50)×U06) showed heterozygous profile of (U06×P50) F1, and the ones with non-lepis wing in BC1F exhibited the homozygous profile of the strain U06. Using a reciprocal BC1M (Using F1 as male to backcross to the recessive parent, that is U06×(U06×P50))cross, we constructed a linkage map of 125.6 cM, and the distance between nlw and the nearest marker cash2p was 11.4 cM.
Key words： Bombyx mori; non-lepis wing; Gene linkage map; SSR; STS
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