荧光原位杂交技术分析栽培种甘薯(Ipomoea batatas cv.Xushu No.18)染色体
收稿日期: 2009-07-17
修回日期: 2009-09-09
网络出版日期: 2010-01-15
基金资助
国家自然科学基金项目(编号：30800698), 徐州市科技计划项目(编号：XM09B036), 江苏省高等学校大学生实践创新训练计划项目(编号：09SSJCX07)和江苏省环洪泽湖生态农业生物技术重点实验室开放基金项目(编号：HZHL0811)资助
FISH analysis of chromosomes of sweet potato(Ipomoea batatas cv.Xushu No.18)
Received date: 2009-07-17
Revised date: 2009-09-09
Online published: 2010-01-15
汤佳立，戚大石，张俞，刘慧娟，孙健英，曹清河，马代夫，李宗芸 . 荧光原位杂交技术分析栽培种甘薯(Ipomoea batatas cv.Xushu No.18)染色体[J]. 遗传, 2010 , 32(2) : 177 -182 . DOI: 10.3724/SP.J.1005.2010.00177
In order to understand the chromosome structure of sweet potato (Ipomoea batatas cv. Xushu 18), molecular cytogenetic analyses were carried out on I. batatas. by using 45S rDNA fluorescence in situ hybridization (45S rDNA-FISH), self genomic in situ hybridization (self-GISH), and silver staining techniques. Twelve, sixteen, and eighteen regions were silver stained in the interphase nucleus of I. batatas. The results of FISH analysis demonstrated 16 or 18 signals with different intensity on chromosomes of I. batatas. Self-GISH analysis showed that the intensive signals on I. batatas mitotic chromosomes were distributed along the chromosomes. However, the signals located in centromeric, subcentromeric, and telomeric regions were stronger and denser than those in other regions.
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