小麦籽粒抗性淀粉含量的分析
收稿日期: 2009-06-08
修回日期: 2009-08-26
网络出版日期: 2010-01-15
基金资助
新疆兵团博士资金项目(编号：ZD2007JC05)资助
Inheritance analysis of resistant starch content in kernels of wheat
Received date: 2009-06-08
Revised date: 2009-08-26
Online published: 2010-01-15
选用3个抗性淀粉含量较高的小麦品种和3个抗性淀粉含量较低的小麦品种按Griffing双列杂交设计配置成15个杂交组合, 以亲本及F1为材料进行了小麦籽粒抗性淀粉含量的遗传规律分析, 旨在为高抗性淀粉含量且综合性状优良的新型保健小麦新品种(系)的选育提供理论依据。结果表明, 在6个小麦品种中, 安农90202和D68-20抗性淀粉含量的一般配合力较好, 能显著地提高杂种后代籽粒抗性淀粉含量。安农90202×04单28和06-5×D68-20组合的特殊配合力最好, 两者特殊配合力效应值显著地高于其他组合。小麦抗性淀粉含量的遗传符合加性-显性模型, 显性程度为超显性。控制抗性淀粉含量的增效等位基因表现为隐性, 且亲本中抗性淀粉含量的增减效等位基因的分布不平衡, 高抗性淀粉含量的亲本中隐性基因数量多于显性基因数量。实验中安农90202和04单28控制抗性淀粉含量的隐性基因较多,而宁春18和新春5号含有的显性基因数量较多。同时研究发现小麦抗性淀粉含量的狭义遗传力中等, 为36.49%。
庞欢，李卫华，张宏斌，王琳，银永安，苑会功，王自布 . 小麦籽粒抗性淀粉含量的分析[J]. 遗传, 2010 , 32(2) : 170 -176 . DOI: 10.3724/SP.J.1005.2010.00170
In this study, three wheat (Triticum aestivum L.) cultivars with high and low levels of resistant starch contents each were selected to obtain 15 F1 combinations from a diallel cross without reciprocals to be used to study the inheritance of resistant starch content. The results of this study are useful to select new wheat cultivar with high level of resistant starch content. Annong 90202 and D68-20 were the best among the wheat cultivars tested for general combining ability of resistant starch content, which significantly increased the resistant starch content in its progenies. The specific combining ability of Annong 90202 × 04 Dan 28 and 06-5 × D68-20 were the best among the F1 combinations, and the values of specific combining ability effects were significantly higher than other combinations. The inheritance of resistant starch content fitted the additive-dominance model, and the degree of dominance was super dominance. The alleles for increasing resistant starch content were recessive. The distribution of alleles for increasing and reducing resistant starch contents in the parental lines was not even. The number of recessive alleles for resistant starch content was greater than the dominant alleles. Annong 90202 and 04 Dan 28 had more recessive genes controlling resistant starch content, while Ningchun 18 and Xinchun 5 had more dominant genes. The narrow sense heritability of resistant starch content was 36.49%.
[1] Englyst HN, Wiggs HS, Cummings JH. Determination of the nonstarch polysaccharides in plant foods by gaslipid chromatography of content sugars as alditol acetates. Analyst, 1982, 107: 307–318.
[2] Asp NQ, Bjock I. Resistant starch. Trends Food Sci Technol, 1992, 3: 111–114.
[3] Heijnen MA, Deurenberg P, Amelsvoort JM, Beynen AC. Replacement of digestible by resistant starch lowers diet-induced thermogenesis in healthy men. Bri J Nutr, 1995, 73: 423–432.
[4] Coudray C, Demigne C, Raysslguier Y. Effects of dietary fibers on magnesium absorption in animals and humans. J Nutr, 2003, 133(1): 1–4.
[5] Sajilata MG, Singhal RS. Specialty starches for snack foods. Carbohyd Polym, 2005, 59: 131–151.
[6] 王竹, 门建华, 杨月欣, 洪洁. 抗性淀粉对大鼠锌营养状况的影响. 营养学报, 2002, 24(2): 166–170.
[7] 王琳, 银永安, 王雪梅, 薛芳, 李卫华. 抗性淀粉及其在春小麦种质资源中含量的测定. 石河子大学学报, 2008, 4(2): 190–194.
[8] Behall KM, Howe JC. Contribution of fiber and resistant starch to metabolizable energy. Am J Clin Nutr, 1995, 62(5 Suppl.): 1158S–1160S.
[9] Eerlingen RC, Delcour JA. Formatting structure and properties of type Ⅲ enzyme resistant starch. Cereal Sci Analysis, 1995, 22: 129–138.
[10] 杨光, 杨波, 丁霄霖. 直链淀粉和支链淀粉对抗性淀粉形成的影响. 食品工业科技, 2008, 29(6): 165–167.
[11] 顾振宇, 樊镇棣, 黄赣辉, 邓丹雯, 余飞. 直链淀粉含量及检测方法与抗性淀粉增抗效应研究. 食品科学, 2008, 29(1): 74–77.
[12] 万志兵. 水稻抗性淀粉含量的研究. 北方水稻, 2008, 38(6): 11–14.
[13] Akerberg A, Liljeberg H, Bjorck I. Effects of amylase/amy- lopectin ratio and baking conditions on resistant starch for-mation and glycaemic indices. J Cereal Sci, 1998, 28: 71–80.
[14] 牟方贵, 闫宗武, 冉瑞林, 滕建勋, 陈永波, 杨朝柱, 李明辉, 吴殿星. 水稻抗性淀粉相关SSR标记的初步研究. 分子植物育种, 2008, 6(3): 432–438.
[15] 王琳, 李卫华, 庞欢, 银永安, 苑会功, 刘磊. 小麦高抗性淀粉含量相关分子标记的筛选与验证. 麦类作物学报, 2009, 29(3): 390–395.
[16] 阮少兰, 刘亚伟, 阮竞兰. 大米抗性淀粉制备工艺研究. 粮食与饲料工业, 2005, (7): 16–17.
[17] 朱昱鹏, 李新华, 刘爱华. 玉米抗牲淀粉的制备及其牲质的研究. 粮油加工, 2005, 5: 79–82.
[18] 刘来福, 毛盛贤, 黄远樟. 作物数量性状遗传. 北京: 农业出版社, 1984.
[19] 李家瑞, 赵伟, 李全梓. Waxy基因的RNA沉默使转基因小麦种子中直链淀粉含量下降. 遗传学报, 2005, 32(8): 846–854.
[20] Kusaba M, Miyahara K, Lida S. Low glutein content 1: a dominant mutation that suooresses the glutein multigene family via RNA silencing in rice. Plant Cell, 2003, 15: 1455–1467.
[21] Palmer KE, Thomson JA, Rybicki EP. Generation of maize cell lines containing autonomously replicating maize streak virus based gene vectors. Arch Virol, 1999, 144(7): 1345–1360.
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