马基因组研究进展
收稿日期: 2009-08-06
修回日期: 2009-10-19
网络出版日期: 2010-03-15
基金资助
国家自然科技平台建设项目(编号：2005DKA21101)和国家自然科学基金项目(编号：30760162)资助
Progress on horse genome project
Received date: 2009-08-06
Revised date: 2009-10-19
Online published: 2010-03-15
各种生物都具有其独特的遗传信息, 深入了解生物体的形成过程以及各种生命活动都离不开基因组的研究成果。由于在世界范围内马具有良好的健康情况记录和详细的系谱记录, 使得马成为生命科学研究中极具价值的模式动物。尽管起步较晚, 但在过去的几年中, 马基因组图谱经历了前所未有的发展。文章主要对近年来马基因组遗传图谱、物理图谱、基因组比较作图以及功能基因组学等研究进展进行了综述, 这些图谱也正是世界各地研究人员用以探寻与马的各种性状(包括全部的健康状况、抗病性能、生殖生育能力、运动性能以及诸如毛色这样的表型特征等)相关基因的重要工具。相信这些研究成果将为马匹疾病预防、诊断和治疗提供新的思路与方法, 并将为马遗传育种提供更好的选配依据。
杨虹，马月辉，李蓓，芒来 . 马基因组研究进展[J]. 遗传, 2010 , 32(3) : 211 -218 . DOI: 10.3724/SP.J.1005.2010.00211
There is unique genetic information belonging to various kinds of living beings. Understanding of the formation process of organisms and a variety of vital movement is associated with the achievements of genome study. As horse has a notable health condition and great record of the genealogy in the world, thus it becomes a valuable model animal for studying life science. Despite of a late start, the map of the horse genome has undergone unprecedented expansion during the last few years. The current progresses of the horse genome, including genetic map, physical map, comparative genomic map, and functional genomics, were reviewed in this paper. The maps are currently used worldwide to discover genes associated with various traits of significance in horse including general health, disease resistance, reproduction, fertility, athletic performance, phenotypic characteristics like coat color, etc. The results are believed to provide new ideas and approaches for prevention, diagnostics, and therapeutic for horses, and also better foundation of breed selection and equine genetic breeding.
Key words： horse; genome map; genome sequencing; comparative genomics; genome analysis
[1] Bowling AT, Breen M, Chowdhary BP, Hirota K, Lear T, Millon LV, Ponce de Leon FA, Raudsepp T, Stranzinger G. International system for cytogenetic nomenclature of the domestic horse. Report of the Third International Com-mittee for the Standardization of the domestic horse karyotype, Davis, CA, USA, 1996. Chromosome Res, 1997, 5(7): 433–443.
[2] Bugno M, S?ota E, Ko?cielny M. Karyotype evaluation among young horse populations in Poland. Schweiz Arch Tierheilkd, 2007, 149(5): 227–232.
[3] Bugno M, Zabek T, Golonka P, Pieńkowska-Schelling A, Schelling C, S?ota E. A case of an intersex horse with 63, X/64, XX/65, XX, del(Y)(q?) karyotype. Cytogenet Genome Res, 2008, 120(1–2): 123–126.
[4] Xu X, Arnason U. The complete mitochondrial DNA se-quence of the horse, Equus caballus: extensive heteroplasmy of the control region. Gene, 1994, 148(2): 357–362.
[5] George M Jr, Ryder OA. Mitochondrial DNA Evolution in the Genus Equus. Mol Biol Evol, 1986, 3(6): 535–546.
[6] Chowdhary BP, Raudsepp T. The horse genome. Genome Dyn, 2006, 2: 97–110.
[7] Tozaki T, Swinburne J, Hirota K, Hasegawa T, Ishida N, Tobe T. Improved resolution of the comparative horse-human map: investigating markers with in silico and linkage map-ping approaches. Gene, 2007, 392(1-2): 181–186.
[8] Penedo MC, Millon LV, Bernoco D, Bailey E, Binns M, Cholewinski G, Ellis N, Flynn J, Gralak B, Guthrie A, Hasegawa T, Lindgren G, Lyons LA, Røed KH, Swinburne JE, Tozaki T. International Equine Gene Mapping Workshop Report: a comprehensive linkage map constructed with data from new markers and by merging four mapping resources. Cytogenet Genome Res, 2005, 111(1): 5–15.
[9] Swinburne JE, Boursnell M, Hill G, Pettitt L, Allen T, Chowdhary B, Hasegawa T, Kurosawa M, Leeb T, Mashima S, Mickelson JR, Raudsepp T, Tozaki T, Binns M. Single linkage group per chromosome genetic linkage map for the horse, based on two three-generation, full-sibling, crossbred horse reference families. Genomics, 2006, 87(1): 1–29.
[10] Raudsepp T, Santani A, Wallner B, Kata SR, Ren C, Zhang HB, Womack JE, Skow LC, Chowdhary BP. A de-tailed physical map of the horse Y chromosome. Proc Natl Acad Sci USA, 2004, 101(25): 9321–9326.
[11] Raudsepp T, Gustafson-Seabury A, Durkin K, Wagner ML, Goh G, Seabury CM, Brinkmeyer-Langford C, Lee EJ, Agarwala R, Stallknecht-Rice E, Schäffer AA, Skow LC, Tozaki T, Yasue H, Penedo MC, Lyons LA, Khazanehdari KA, Binns MM, MacLeod JN, Distl O, Guérin G, Leeb T, Mickelson JR, Chowdhary BP. A 4,103 marker integrated physical and comparative map of the horse genome. Cytogenet Genome Res, 2008, 122(1): 28–36.
[12] Looft C, Paul S, Philipp U, Regenhard P, Kuiper H, Distl O, Chowdhary BP, Leeb T. Sequence analysis of a 212 kb defensin gene cluster on ECA 27q17. Gene, 2006, 376(2): 192–198.
[13] Müller D, Kuiper H, Mömke S, Böneker C, Drögemüller C, Swinburne JE, Binns M, Chowdhary BP, Distl O. Physical mapping of the ATP2A2 gene to equine chromosome 8p14→p12 by FISH and confirmation by linkage and RH map-ping. Cytogenet Genome Res, 2006, 114(1): 94G.
[14] Perelygin AA, Lear TL, Zharkikh AA, Brinton MA. Com-parative analysis of vertebrate EIF2AK2 (PKR) genes and assignment of the equine gene to ECA15q24-q25 and the bovine gene to BTA11q12-q15. Genet Sel Evol, 2006, 38(5): 551–563.
[15] Klukowska-Rötzler J, Bugno M, Sander P, Slota E, Dolf G, Chowdhary BP, Leeb T, Gerber V. Chromosomal assign-ment of the two candidate genes (EGFR, CLCA1) for equine recurrent airway obstruction (RAO) by FISH and RH mapping. Hereditas, 2006, 143(2006): 138–141.
[16] Prause A, Guionaud C
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