云南傣族、汉族HLA-G基因14 bp插入/缺失多态性研究
收稿日期: 2009-11-03
修回日期: 2009-12-29
网络出版日期: 2010-05-24
基金资助
云南省应用基础研究重点项目(编号：2008CC021)和美国中华医学基金会资助项目(编号：04-805)资助
Analysis of the 14 bp insertion/deletion polymorphism in human leukocyte antigen-G gene in Chinese Dai and Han ethnic groups in Yunnan Province
Received date: 2009-11-03
Revised date: 2009-12-29
Online published: 2010-05-24
14 bp插入/缺失多态性是指存在于HLA-G基因第8外显子3′非翻译区(3′UTR)的一个插入/缺失多态, 因其能影响HLA-G mRNA的稳定性, 并进一步影响HLA-G蛋白的翻译而受到广泛关注。文章采用PCR和电泳技术对云南傣族和汉族两个人群进行了HLA-G基因14 bp插入/缺失多态性的检测分析, 结果显示傣族和汉族人群+14 bp等位基因频率分别为31.97%和40.87%, +14 bp/+14 bp基因型频率分别为8.20%和17.31%, +14 bp/-14 bp基因型频率分别为47.54% 和47.11%。与国内外已报道的其他人群的数据比较表明: 云南汉族群体中HLA-G基因14 bp插入/缺失的分布与其他群体相似; 傣族则有自己独特的基因型和等位基因分布特点, 推测其可能受到了遗传漂变的作用, 但不排除自然选择作用的影响。
关键词： 随机遗传漂变; 自然选择; HLA-G基因; 14 bp插入/缺失多态性
陈金宝，史磊，姚宇峰，史荔，于亮，林克勤，陶玉芬，易文，黄小琴，孙浩，褚嘉祐 . 云南傣族、汉族HLA-G基因14 bp插入/缺失多态性研究[J]. 遗传, 2010 , 32(6) : 577 -582 . DOI: 0.3724/SP.J.1005.2010.00577
A 14 bp insertion/deletion polymorphism (+14 bp/-14 bp) in exon 8 of HLA-G gene has been investigated extensively because this polymorphism was associated with HLA-G mRNA stability and could influence HLA-G mRNA expression. In the present study, we genotyped 14 bp insertion/deletion polymorphisms in the HLA-G gene in Dai and Han populations in Yunnan Province. The frequencies of +14 bp in Dai and Han were 31.97% and 40.87%, respectively. The frequency of +14 bp/+14 bp in Dai was lower (8.20%) than that of Han (17.31%). Nevertheless, the frequency of +14 bp/-14 bp was similar in both populations (47.54% in Dai and 47.11% in Han). When compared with other populations, the frequency of 14 bp insertion/deletion of Han was similar to others, whereas the Dai showed significent difference from others and presented it’s specific characteristic. We assumed that the distributaion characteristic of 14 bp insertion/deletion in Dai might be the results of random genetic drift, but the role of natural selection cannot be excluded.
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