DNA技术在海洋食品物种鉴定中的应用
收稿日期: 2009-09-27
修回日期: 2010-01-03
网络出版日期: 2010-05-24
基金资助
广东海洋大学博士启动基金项目(编号：0712130)资助
DNA-based methods for identification of seafood species
Received date: 2009-09-27
Revised date: 2010-01-03
Online published: 2010-05-24
随着分子生物学技术的发展, 海洋食品物种鉴定方法由原来的蛋白质水平深入到了DNA水平。目前应用于海洋食品物种鉴定的DNA技术主要是FINS(Forensically informative nucleotide sequencing)、PCR-RFLP和物种特异性PCR标记技术等, 能够实现对新鲜、冰冻、腌制或灌装食品物种进行鉴定, 而对混合样本的鉴定及量化分析是尚待解决的一个问题。基因数据库对物种鉴定的影响也越来越大, 是海洋加工食品物种鉴定可利用的另一种重要信息资源。文章综述了DNA技术在海洋食品物种鉴定中的应用研究进展, 并展望DNA技术在海洋食品检测中的发展趋势。
张丽，张良，刘书成，张义军，韩艺 . DNA技术在海洋食品物种鉴定中的应用[J]. 遗传, 2010 , 32(6) : 555 -560 . DOI: 10.3724/SP.J.1005.2010.00555
With the development of molecular biotechnology, methods for identification of seafood species are developed from protein to DNA. At present, the main DNA-based methods for species identification are FINS, PCR-RFLP, and specific-PCR, which have been used to identify the species of fresh, frozen, and pickled or canned seafood. However, qualitative and quantitative methods for identification of the mixed seafood species remain to be resolved. The gene databases play an important role in identifying species and are valuable information resources for identification of seafood species. In this paper, recent progresses of major DNA-based methods for identification of seafood species are reviewed and the perspec-tives of this field are discussed.
Key words： DNA biotechnology; seafood; species identification
[1] Moretti VM, Turchini GM, Bellagamba F, Caprino F. Trace ability issues in fishery and aquaculture products. Vet Res Commun, 2003, 27(Suppl. 1): 497–505.
[2] Civera T. Species identification and safety of fish products. Vet Res Commun, 2003, 27(Suppl. 1): 481–489.
[3] Woolfe M, Primrose S. Food forensics: using DNA tech-nology to combat misdescription and fraud. Trends Bio-technol, 2004, 22(5): 222–226.
[4] Chapela MJ, Sotelo CG, Perez-Martin RI, Pardo MA, Perez-Villareal B, Gilardi P, Riese J. Comparison of DNA extraction methods from muscle of canned tuna for species identification. Food Control, 2007, 18(10): 1211–1215.
[5] Santaclara FJ, Cabado AG, Vieites JM. Development of a method for genetic identification of four species of an-chovies: E. encrasicolus, E. anchoita, E. ringens and E. japonicus. Eur Food Res Technol, 2006, 223(5): 609–614.
[6] Calo-Mata P, Sotelo CG, Perez-Martin RI, Rehbein H, Hold GL, Russell VJ, Pryde S, Quinteiro J, Rey-Mendez M, Rosa C, Santos AT. Identification of gadoid fish spe-cies using DNA based techniques. Eur Food Res Technol, 2003, 217(3): 259-264.
[7] Sotelo CG, Calo-Mata P, Chapela MJ,Perez-Martin RI, Rehbein H, Hold GL, Russell VJ, Pryde S, Quinteiro J, Izquierdo M, Rey-Mendez M, Rosa C, Santos AT. Identi-fication of flatfish (Pleuronectiforme) species using DNA-based techniques. J Agric Food Chem, 2001, 49(10): 4562-4569.
[8] Rehbein H, Sotelo CG, Perez-Martin RI, Chapela-Garrido MJ, Hold GL, Russell VJ, Pryde S, Santos AT, Rosa C, Quinteiro J, Rey-Mendez M. Differentiation of raw or processed eel by PCR-based techniques: restriction frag-ment length polymorphism analysis (RFLP) and single strand conformation polymorphism analysis (SSCP). Eur Food Res Technol, 2002, 214(2): 171-177.
[9] Teletchea F, Maudet C, Hanni C. Food and forensic mo-lecular identification: update and challenges. Trends Biotechnol, 2005, 23(7): 359-366.
[10] Akasaki T, Yanagimoto T, Yamakami K, Tomonaga H, Sato S. Species identification and PCR-RFLP analysis of cytochrome b gene in cod fish (order Gadiformes) prod-ucts. J Food Sci, 2006, 71(3): C190-195.
[11] Chakraborty A, Aranishi F, Iwatsuki Y. Molecular identi-fication of hairtail species (Pisces: Trichiuridae) based on PCR-RFLP analysis of the mitochondrial 16S rRNA gene. J Appl Genet, 2005, 46(4): 381-385.
[12] Chow S, Nohara K, Tanabe T, Itoh T, Tsuji S, Nishikawa Y, Uyeyanagi S, Uchikawa K. Genetic and morphological identification of larval and small juvenile tunas (Pisces: Scombridae) caught by a mid-water trawl in the western Pacific. Bull Fish Res Agen, 2003, (8): 1-14.
[13] Clarke SC, Magnussen JE, Abercrombie DL, McAllister MK, Shivji MS. Identification of shark species composi-tion and proportion in the Hong Kong shark fin market based on molecular genetics and trade records. Conserv Biol, 2006, 20(1): 201-211.
[14] Moran P, Garcia-Vazquez E. Identification of highly prized commercial fish using a PCR-based methodology. Biochem Mol Biol Educat, 2006, 34(2): 121-124.
[15] Bartlett SE, Davidson WS. FINS (forensically informative nucleotide sequencing): a procedure for identifying the animal origin of biological specimens. Biotechniques, 1992, 12(3): 408–411.
[16] Chapela MJ, Sotelo CG, Calo-Mata P, Perez-Martin RI, Rehbein H, Hold GL, Quinteiro J, Rey-Mendez M, Rosa C, Santos AT. Identification of cephalopod species (Ommas-trephidae and Loliginidae) in seafood products by foren-sically informative nucleotide sequencing (FINS). J Food Sci, 2002, 67(5): 1672-1676.
[17] Pepe T, Trotta M, di Marco I, Cennamo P, Anastasio A, Cortesi ML. Mitochondrial cytochrome b DNA sequence variations: an approach to fish species identification in p
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