黄瓜单性花决定基因M的表达分析
收稿日期: 2009-10-11
修回日期: 2010-03-04
网络出版日期: 2010-05-24
基金资助
国家自然科学基金项目(编号: 30871707), 国家“863”项目(编号: 2008AA10Z150), 农业部“948”项目(编号: 2008-Z42)和上海市重点学科建设项目(编号: B209)资助
Expression analysis of the unisex-determine gene M in cucumber
Received date: 2009-10-11
Revised date: 2010-03-04
Online published: 2010-05-24
植物成花性型决定过程涉及多种机制, 而单一基因位点控制非完全花(单性花)的发育为葫芦科植物所特有, 其中以黄瓜(Cucumis sativus L.)和甜瓜(C. melo L.)研究最深入。文章利用两对黄瓜近等基因系材料, 结合定量PCR技术, 研究黄瓜单性花决定基因M(CsACS2, GenBank登陆号: FJ529216)的表达情况。利用药剂(AgNO3和艾维激素)处理不同基因型黄瓜材料, 研究在内源乙烯作用被干扰的情况下CsACS2基因的表达变化。结果表明: 内源乙烯可以诱导CsACS2基因的表达。在正常生长条件下, 这种内源乙烯可能来自CsACS1G基因(F基因)或CsACS2基因(M基因)本身, 而后一种情况可能涉及一种正回馈激活机制。
陶倩怡，李征，何欢乐，潘俊松，蔡润 . 黄瓜单性花决定基因M的表达分析[J]. 遗传, 2010 , 32(6) : 632 -638 . DOI: 10.3724/SP.J.1005.2010.00632
Sex determination in plants has various machnisam; however, a single gene locus controlling unisexual expression is unique in the Cucurbitaceae plants, particularly cucumber (Cucumis sativus L.) and melon (Cucumis melo L.). In this study, with quantitative RT-PCR method, two sets of near-isogenic lines (NILs) were used to analyze the expression pattern of gene CsACS2 (GenBank accession number FJ529216). Additionally, chemical applications (AgNO3 and AVG) were used to investi-gate the effect of the plant endogenous ethylene on CsACS2 expression. Expression analysis reveals that endogenous ethylene, which might be derived from F or M itself, could activate the expression of M gene.
Key words： Cucumis sativus L.; sex determination; M gene; CsACS2; expression analysis
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