北极狐GHR基因单核苷酸多态性及其与生长性状的相关性分析
Association analysis between SNPs of the growth hormone receptor gene and growth traits in arctic fox
Received date: 2009-09-15
Revised date: 2010-02-27
Online published: 2010-05-24
文章采用单链构象多态性(PCR-SSCP)和DNA测序的方法检测了北极狐生长激素受体(Growth hormne receptor, GHR)基因的单核苷酸多态性(SNPs), 并针对该群体的特点建立合适的统计分析模型, 对GHR基因多态性与生长性状的相关性进行了分析。结果表明, 在北极狐GHR基因的外显子1和外显子5上发现了4个多态位点, 分别为5′UTR上的G3A和外显子1上的C99T突变, 外显子5上的T59C和G65A突变; GHR基因G3A和C99T多态性与母狐的体重性状显著相关(P<0.05), T59C和G65A多态性与公狐的体重性状显著相关(P<0.05), 与母狐的皮张长度性状极显著相关(P<0.01)。因此, 可以利用以上点突变对北极狐的体重及皮张长度性状进行标记辅助选择研究, 以达到快速选育出快大、优质的北极狐的目的。
杜智恒，刘宗岳，白秀娟 . 北极狐GHR基因单核苷酸多态性及其与生长性状的相关性分析[J]. 遗传, 2010 , 32(6) : 599 -605 . DOI: 10.3724/SP.J.1005.2010.00599
Using single-strand conformation polymorphism (PCR-SSCP) and DNA sequencing, single nucleotide polymorphisms (SNPs) of growth hormone receptor (GHR) gene were detected in an arctic fox population. Correlation analysis between GHR polymorphisms and growth traits were carried out using the appropriate model. Four SNPs, G3A in the 5′UTR, C99T in the first exon, T59C and G65A in the fifth exon were identified on the arctic fox GHR gene. The G3A and C99T polymorphisms of GHR were associated with female fox body weight (P<0.05) and the T59C and G65A polymorphisms of GHR were associated with male fox body weight (P<0.05) and the skin length of the female fox (P<0.01). Therefore, marker assistant selection on body weight and skin length of arctic foxes using these SNPs can be applied to get big and high quality arctic foxes.
Key words： arctic fox; GHR gene; SNP; growth trait
[1] Graphodatsky AS, Yang F, Serdukova N, Perelman P, Zhdanova NS, Ferguson-Smith MA. Dog chromo-some-specific paints reveal evolutionary inter and in-trachromosomal rearrangements in the American mink and human. Cytogenet Cell Genet, 2000, 90(3–4): 275–278.
[2] 胡晓航. 银狐生长激素基因克隆与基因表达研究[学位论文]. 东北林业大学, 2005.
[3] 孙逊, 朱尚权. 生长激素的结构与功能. 国外医学生理、病理科学与临床分册, 1999, 19(1): 6–9.
[4] Falaki M, Gengler N, Sneyers M, Prandi A, Massart S, Formigoni A, Burny A, Portetelle D, Renaville R. Relationships of polymorphisms for growth hormone and growth hormone receptor genes with milk production traits for Italian Holstein-Friesian bulls. J Dairy Sci, 1996, 79(8): 1446–1453.
[5] Blott S, Kim J, Moisio S, Schmidt-Kuntzel A, Cornet A, Berzi P, Cambisano N, Ford C, Grisard B, Johnson D, Wong J, Vilkki J, Georges M, Farnir F, Coppieters W. Molecular dissection of a quantitative trait locus: a phenylalanine-to-tyrosine substitution in the transmem-brane domain of the bovine growth hormone receptor is associated with a major effect on milk yield and composi-tion. Genetics, 2003, 163(1): 253–266.
[6] Maj A, Oprzadek J, Dymhicki E, Zwierzchowski L. Asso-ciation of the polymorphism in the 5′-noncoding region of the bovine growth hormone receptor gene with meat production traits in Polish Black-white cattle. Meat Sci, 2006, 72: 539–544.
[7] Godowski PJ, Leung DW, Meacham LR, Galgani JP, Hellmiss R, Keret R, Rotwein PS, Parks JS, Laron Z, Wood WI. Characterization of the human growth hormone receptor gene and demonstration of a partial gene deletion in two patients with Laron-type dwarfism. Proc Natl Acad Sci USA, 1989, 86(20): 8083–8087.
[8] 杜智恒, 宁方勇, 杨春山, 白秀娟. 北极狐GHR基因cDNA的克隆及序列分析. 兽类学报, 2009, 30(1): 65–73.
[9] Curi RA, Oliveira HN, Silveira AC, Lopes CR. Effects of polymorphism microsattellites in the regulatory region of IGF1 and GHR on growth and carcass traits in beef cat-tle. Anim Genet, 2005, 136(1): 58–62.
[10] Hale CS, Herring WO, Shibuya H, Lucy MC, Lubahn DB, Keisler DH, Johnson GS. Decreased growth in Angus steers with a short TG-microsatellite allele in the P1 pro-moter of the growth hormone receptor gene. Animl Sci, 2000, 78(8): 2099–2104.
/
| 〈 |
|
〉 |
www.chinagene.cn
备案号：京ICP备09063187号-4
总访问:,今日访问:,当前在线:
