早期胚胎的死亡会给畜牧业的发展带来巨大的经济损失, 特别是对于母牛生产来说更是如此, 因此研究早期胚胎发育过程具有极为重要的价值。文章从公共数据库GEO中选取了关于牛早期胚胎发育过程的一套基因表达谱数据, 通过显著性分析及聚类分析来研究牛早期胚胎发育过程中不同时期的基因组表达模式。结果表明: 整个牛早期胚胎发育过程大致可划分为3组不同的基因组表达模式阶段; 同时, 差显基因在不同时期表达量的波动情况表明8-细胞期和16-细胞期在牛的整个早期胚胎发育过程的重要性。另外, 通过进一步的功能注释和通路分析表明在牛胚胎早期发育不同阶段时期存在着一些重要因子及相关通路。

The death of early embryo development will bring huge economic losses in animal husbandry, especially for dairy production of cattle. Thus, it has an extremely important value to study the process of early embryonic development. In our study, one dataset of gene expression was chosen from the public database of GEO, which was related to bovine preimplantation development. Significance and clustering analyses were used to study the genome expression patterns of each stage during bovine preimplantation development. The whole process was assigned to three main groups. The waves of significant gene expression in each stage indicated that both 8-cell and 16-cell stages played an important role during the process of bovine preimplantation development. Further functional analysis and pathway analysis revealed that some major factors and related pathways are required for distinctive developmental stages.