研究报告

巴西橡胶树SSR遗传图谱的构建

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  • 1. 中国热带农业科学院热带作物生物技术研究所, 海口 571101; 2. 中国热带农业科学院橡胶研究所, 儋州 571737; 3. 琼州学院, 五指山 572200; 4. 海南大学农学院,海口 570228

收稿日期: 2009-10-16

  修回日期: 2010-01-05

  网络出版日期: 2010-08-23

基金资助

中央级公益性科研院所基本科研业务费项目(编号:ITBBZD0715)资助

Construction of genetic linkage map for rubber tree (Hevea brasiliensis)based on SSR markers

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  • 1. Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Science, Haikou 571101, China; 2. Rubber Cultivation Research Institute, Chinese Academy of Tropic Agricultural Science, Danzhou 571737, China; 3. Qiongzhou University, Wuzhishan 572200, China; 4. Agricultural College, Hainan University, Haikou 570228, China

Received date: 2009-10-16

  Revised date: 2010-01-05

  Online published: 2010-08-23

摘要

以热研88-13×IAN873的94个F1群体为试材, 利用简单序列重复(Simple sequence repeat, SSR)标记, 采用FsLinkageMAP 1.0软件, 构建了巴西橡胶树热研88-13×IAN873的遗传连锁图谱。从441对SSR引物中筛选出160对具有多态信息的引物, 在分离群体中共检测到206个多态性位点, 176个位点用于遗传图谱的构建; χ2检验结果显示, 有147个位点符合1:1分离比例, 有12个符合1:2:1分离比例, 有17个符合1:1:1:1的分离比例, 共有13个偏分离位点, 偏分离率低(7.38%); 91个SSR位点被分为18个连锁群, 覆盖橡胶树基因组1 937.06 cM, 每个连锁群包含2~16个位点, 标记间的平均距离为21.29 cM。

本文引用格式

冯素萍,李维国,于飞,王静毅,武耀廷 . 巴西橡胶树SSR遗传图谱的构建[J]. 遗传, 2010 , 32(8) : 857 -863 . DOI: 10.3724/SP.J.1005.2010.00857

Abstract

Out of 260 polymorphic loci screened from a total of 441 pairs of EST-SSR and genomic-SSR primers, 176 were used for constructing the genetic map of rubber tree (Hevea brasiliensis) by an F1 segregating population including 94 progenies from the cross Reyan 88-13×IAN873. Chi-square test carried out on the polymorphic loci used in constructing the map showed that 147 loci followed a segregation ratio of 1:1 and 12 loci followed a ratio of 1:2:1 and 17 loci followed a ratio of 1:1:1:1. Only 13 (7.38%) loci were distorted from the Mendelian ratio. The genetic linkage map consisted of 91 marker loci in 18 linkage groups and covered 1 937.06 cM with an average genetic distance of 21.29 cM between adjacent markers. The largest linkage group consisted of 16 marker loci, while the smallest one contained only 2 marker loci.

参考文献

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