采用长PCR扩增的线粒体DNA和引物步移法, 测定并注释了中国特有鸟类—黑尾地鸦(Podoces hendersoni)的线粒体基因组全序列。黑尾地鸦的mtDNA序列全长16 867 bp, GenBank登录号GU592504。基因含量和排列次序与原鸡的一致, 包含13个蛋白编码基因、22个tRNA、2个rRNA和1个控制区(D-loop)。除COI基因以GTG作为起始密码子外, 其余12个蛋白质编码基因均以典型ATG密码子起始。11个蛋白编码基因以完全终止密码子TAA、AGG或AGA终止, COIII和ND4基因终止密码子为不完整的T。tRNASer(AGY)的DHU臂缺失, tRNALeu(CUN)的反密码子环由9个碱基构成, 而不是标准的7个碱基。其余的20个tRNA基因的二级结构均属典型的三叶草结构。预测了rRNA的二级结构, 其中, 12S rRNA二级结构包含4个结构域, 43个茎环结构; 16S rRNA的二级结构包含6个结构域, 55个茎环结构。此外, 在其他鸟类控制区中所发现的F-box、D-box、C-box、B-box、Bird similarity-box和CSB1-box也同样存在于黑尾地鸦中。
The complete mitochondrial genome of a China endemic bird, Podoces hendersoni, was sequenced using La-PCR and conserved primer walking approaches. The mtDNA seqnence is 16 867 bp in length and deposited in GenBank with accession number GU592504. The mitochondrial genomic organization of P. hendersoni is the same with that in chicken, which contains 13 protein coding genes (PCGs), 22 tRNA, 2 rRNA, and a control region. Except for COI gene, which uses GTG as the initiation codon, all other 12 PCGs of the P. hendersoni mtDNA start with the typical ATG codon. Codons TAA, AGG, and AGA were used in 11 PCGs as usual termination codons; however, the COIII and ND4 had incomplete termination codon T. The secondary structures of 20 tRNAs formed typical cloverleaf, except for tRNASer (AGY) that had an absence of the DHU arm and tRNALeu (CUN) in which anticodon-loop consisted of 9 bases, rather than the standard 7 bases. The secondary structures of rRNA were predicted. There are 4 domains, 43 helices structures in 12S rRNA, and 6 domains, 55 helices structures in 16S rRNA. Besides, F-box, D-box, C-box, B-box, Bird similarity-box and CSB1-box, which were found in the control region of other birds, also existed in the P. hendersoni.
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