To investigate the conserved homologs of filamentous ascomycetes genomes, the local fungal genome database used in this analysis was established, which consisted of 31 latest and complete genome data publicly available on the Internet. An expectation value cutoff of 0.1 was used to identify significant hits. Each complete gene set of the query genome Aspergillus nidulans genome with 10 560 annotated genes was splitted into individual FASTA files with Seqverter and then compared separately against each filamentous ascomycete genome using Standalone BLASTN. The result indicated that the number of matches reflected the evolutional relationships of the filamentous ascomycetes analysed. Of 10 560 genes in Aspergillus nidulans genome, 924 had match sequences with other 30 filamentous ascomycetes ones. The number of homology sequences were 6, 3, 6, and 6 at E-values in the range of 10-5 to 0.1, 10-30 to 10-5, 10-100 to 10-5 and 0 to 10-100, respecively. Six homologs at E-values ranging from 10-5 to 0.1 and 3 at E-values ranging from 10-30 to 10-5 were variable, while the 6 at E-values ranging from 0 to 10-100 were highly conserved based on the alignments using ClustalX. Six homologs were relatively conserved at E-values in the range of 10-100 to 10-30, which can be used in phylogeny of these filamentous ascomycetes in this study.
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