环形染色体构象俘获(Circular chromosome conformation capture, 4C)是一种高通量研究细胞染色体相互作用、空间构象的技术。文章通过模拟的4C样品, 优化反向PCR条件, 建立了高效、特异的扩增方法, 对4C克隆筛选等后续步骤进行严格的质量控制并对该方法的可行性进行了实例验证。该4C-克隆筛选方法作为4C方法的质量控制标准, 具有重要的指导和监测作用。
Circular chromosome conformation capture (4C) can be used to analyze the high-resolution interaction map of cis-regulatory elements in genome-wide studies. In this study, we optimized the condition of PCR with the mimical 4C sample in order to establish a specific and efficient assay, which was validated practically. This 4C-clone screening assay can be used as the quality control in the application of 4C assay.
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