文章通过对东北梅花鹿(Cervus nippon hortulorum)鹿茸尖端组织cDNA文库随机测序获得了906条高质量ESTs, 906条ESTs拼接后代表了701个Unigenes, 其中包括重叠群86个, 单拷贝615个。Blast分析显示具已知和推测功能的基因580个(82.7%), 通过Gene Ontology (GO)分类对获得的580个功能基因进行了包括分子功能、生物过程和细胞组分在内的3个层次的功能注释, 并根据BLAST的注释结果及进一步的筛选与分析, 共得到39条与鹿茸尖端组织生长发育相关的基因。cDNA文库的构建和ESTs分析填补了鹿科动物在NCBI公共数据库上基因组信息的空白, 并为科学的开发和利用梅花鹿资源提供了重要的理论依据。
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