转基因生物安全性问题已引起了公众的普遍担忧, 严重制约了转基因技术成果的推广应用。近年来, 研究者探索了不同的技术策略来解决转基因生物的安全性问题, 安全转基因技术成为当前转基因研究的热点之一。文章介绍并评价了几种主要的安全转基因技术, 包括无选择性标记基因技术、安全标记基因技术、叶绿体转化技术、终止子技术、雄性不育技术、外源基因删除技术。其中, 外源基因删除技术为实现转基因生物的安全应用展示了诱人的前景。最后, 对探索新的安全转基因技术策略保障转基因生物安全提出了建议。
The biosecurity of transgenic organism has been widely concerned and extremely restricted its application. Recently, many technological strategies have been developed to ensure its biosecurity. Thus, transgene containment technologies have become one of the hotspots in current transgenic research. In this paper, several transgene containment technologies, such as marker-free transgenic technology, safety marker transgenic technology, chloroplast transgenic technologies, terminator technology, male sterility technology, and ‘GM-gene-deletor’technology were reviewed and evaluated. ‘GM-gene-deletor’ technology, as one of these technologies, demonstrated a prosperous future for safe application of transgenic organisms. Finally, the strategies for developing new transgene containment technologies have been suggested.
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