综述

fC31整合酶系统介导的位点特异性整合研究进展

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  • 上海交通大学附属儿童医院, 上海交通大学医学遗传研究所, 上海 200040

收稿日期: 2011-01-17

  修回日期: 2011-04-06

  网络出版日期: 2011-06-25

基金资助

转基因生物新品种培育科技重大专项(编号:2009ZX08010-018B)

Progress of fC31 integrase system in site-specific integration

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  • Children’s Hospital of Shanghai, Institute of Medical Genetics, Shanghai JiaoTong University, Shanghai 200040, China

Received date: 2011-01-17

  Revised date: 2011-04-06

  Online published: 2011-06-25

摘要

来源于链霉菌(Streptomyces)噬菌体fC31的整合酶可介导链霉菌附着位点(attB)和噬菌体附着位点(attP)之间的同源重组, 这种重组亦可在多种动植物细胞内进行; 而且, 该整合酶还可介导含attB位点的载体以位点特异性方式整合于多种真核生物基因组内的假attP位点, 并使转基因持续高效表达。因此, fC31整合酶在基因修饰、基因治疗及转基因动物研制等方面得到了广泛的应用。文章就近年来fC31整合酶整合规律、提高效率方面的改进及安全性等相关领域的研究进展进行了综述。

本文引用格式

马晴雯 . fC31整合酶系统介导的位点特异性整合研究进展[J]. 遗传, 2011 , 33(6) : 567 -575 . DOI: 10.3724/SP.J.1005.2011.00567

Abstract

Integrase of phage fC31 catalyses the homologous recombination between Streptomyces attachment site attB and the phage attachment site attP. Meanwhile, this integrase can mediate integration of attB-containing donor plasmids into the pseudo attP sites in eukaryotic genomes by a site-specific manner and resulting long-term and robust expression of integrated genes. Nowadays, fC31 integrase system is becoming a potential tool for genome modification, gene therapy and transgenic research. Recent progress of fC31 integrase system in integration mode in mammalian genomes, efficiency improvement and researches concerned on transgenic safety were summarized in this review.

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