综述

转基因番茄的研究现状及其产业化

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  • 1. 东北农业大学园艺学院, 哈尔滨 150030 2. 东北农业大学生命科学学院, 哈尔滨 150030

收稿日期: 2010-11-16

  修回日期: 2011-06-16

  网络出版日期: 2011-09-25

基金资助

教育部高等学校博士学科点专项科研基金(编号:20092325110001)和教育部科学技术研究重点项目(编号:211043)资助

Current status and industrialization of transgenic tomatoes

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  • 1. Northeast Agricultural University, College of Horticulture, Harbin 150030, China 2. Northeast Agricultural University, College of Life Sciences, Harbin 150030, China

Received date: 2010-11-16

  Revised date: 2011-06-16

  Online published: 2011-09-25

摘要

文章综述了近年来国内外番茄转基因研究的进展, 主要包括抗病虫害、抗逆、抗除草剂、耐储运、品质改良、雄性不育等方面的转基因番茄所获得的成果, 并且阐述了利用转基因番茄生产异源蛋白的研究进展。同时对番茄的产业化现状及存在的问题进行了分析, 并就现状对我国今后转基因番茄研究和产业化的前景进行了展望。

本文引用格式

王傲雪,陈秀玲 . 转基因番茄的研究现状及其产业化[J]. 遗传, 2011 , 33(9) : 962 -974 . DOI: 10.3724/SP.J.1005.2011.00962

Abstract

In this review, the progress in transgenic tomatoe research, including disease and insect resistance, herbicide resistance, stress tolerance, long-term storage, quality improvement, and male sterility, were described. The recent researches on producing heterologous proteins using transgenic tomatoes were also reviewed. Furthermore, the industrialization status and problems of transgenic tomatoes were analyzed and the prospects of both research and industrialization in transgenic tomatoes were discussed.

参考文献

[1] Powell AP, Nelson RS, De B, Hoffmann N, Rogers SG, Fraley RT, Beachy RN. Delay of disease development in transgenic plants that express the tobacco mosaic virus coat protein gene. Science, 1986, 232(4751): 738-743.
[2] 周北雁, 李毅, 陈章良. 北京大学的抗病毒转基因作物. 生物技术通报, 1999, (3): 42-45.
[3] Kaniewski W, Ilardi V, Tomassoli L, Mitsky T, Layton J, Barba M. Extreme resistance to cucumber mosaic virus (CMV) in transgenic tomato expressing one or two viral coat proteins. Mol Breed, 1999, 5(2): 111-119.
[4] Zrachya A, Kumar PP, Ramakrishnan U, Levy Y, Loyter A, Arazi T, Lapidot M, Gafni Y. Production of siRNA targeted against TYLCV coat protein transcripts leads to silencing of its expression and resistance to the virus. Transgenic Res, 2007, 16(3): 385-398.
[5] Tamarzizt HB, Chouchane SG, Lengliz R, Maxwell DP, Marrakchi M, Fakhfakh H, Gorsane F. Use of tomato leaf curl virus (TYLCV) truncated Rep gene sequence to engineer TYLCV resistance in tomato plants. Acta Virol, 2009, 53(2): 99-104.
[6] 朱秋菊. 转核酶基因、反义和正义CMV-CP基因番茄的抗黄瓜花叶病毒(CMV)研究
[学位论文]. 北京: 中国科学院研究生院(植物研究所), 2005.
[7] Itaya A, Bundschuh R, Archual AJ, Joung JG, Fei ZJ, Dai XB, Zhao PX, Tang YH, Nelson RS, Ding B. Small RNAs in tomato fruit and leaf development. Biochim Biophys Acta, 2008, 1779(2): 99-107.
[8] Yin ZJ, Li CH, Han XL, Shen FF. Identification of con-served microRNAs and their target genes in tomato (Lycopersicon esculentum). Gene, 2008, 414(1-2): 60-66.
[9] Bazzini AA, Asís R, González V, Bassi S, Conte M, Soria M, Fernie AR, Asurmendi S, Carrari F. miSolRNA: A tomato micro RNA relational database. BMC Plant Biol, 2010, 10(1): 1-7.
[10] Zhang XH, Li HX, Zhang JH, Zhang CJ, Gong PJ, Ziaf K, Xiao FM, Ye ZB. Expression of artificial microRNAs in tomato confers efficient and stable virus resistance in a cell-autonomous manner. Transgenic Res, 2011, 20(3): 569-581.
[11] Jongedijk E, Tigelaar H, Vanroekel JSC, Bresvloemans SA, Dekker I. Synergistic activity of chitinases and β-1,3- glucanases enhances fungal resistance in transgenic tomato plants. Euphytica, 1995, 85(1-3): 173-180.
[12] Chen SC, Liu AR, Wang F H, Ahammed G J. Combined overexpression of chitinase and defensin genesin transgenic tomato enhances resistance to Botrytis cinerea. Afr J Biotechnol, 2009, 8(20): 5182-5188.
[13] Thomzik JE, Stenzel K, Stöcker R, Schreier PH, Hain R, Stahl DJ. Synthesis of a grapevine phytoalexin in transgenic tomatoes (Lycopersicon esculentum Mill.) conditions resistance against Phytophthora infestans. Physiol Mol Plant Pathol, 1997, 51(4): 265-278.
[14] Khan RS, Nakamura I, Mii M. Development of disease-resistant marker-free tomato by R/RS site-specific recombination. Plant Cell Rep, 2011, 30(6): 1041-1053.
[15] Tai TH, Dahlbeck D, Clark ET, Gajiwala P, Pasion R, Whalen MC, Stall RE, Staskawicz BJ. Expression of the Bs2 pepper gene confers resistance to bacterial spot disease in tomato. Proc Natl Acad Sci USA, 1999, 96(24): 14153-14158.
[16] Jan PS, Huang HY, Chen HM. Expression of a synthesized gene encoding cationic peptide cecropin B in transgenic tomato plants protects against bacterial diseases. Appl Environ Microbiol, 2010, 76(3): 769-775.
[17] Balaji V, Mayrose M, Sherf O, Jacob-Hirsch J, Eichenlaub R, Iraki N, Manulis-Sasson S, Rechavi G, Barash I, Sessa G. Tomato transcriptional changes in response to Clavibacter michiganensis subsp. michiganensis reveal a role for ethylene in disease development. Plant Physiol, 2008, 146(4): 1797-1809.
[18] Rezk AAS, Abdallah NA, Abdel AM, Nakhla MK, Mazyad HM, Maxwell DP. Transgene-mediated RNA silencing of TYLCV genes affecting the accumulation of viral DNA in plants. Arab J Biotech, 2006, 9(1): 143-158.
[19] Koshino-Kimura Y, Takenaka K, Domoto F, Ohashi M,
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