肌肉增强因子2(Myocyte enhancer factor 2, MEF2)是MADS(MCM1, agamous, deficiens和serum response factor)家族成员之一, 在动物发育过程中起到重要的调节作用。为了进一步了解其调控的复杂性, 本文根据NCBI中已有的人类MEF2相关数据, 应用ExPASy在线序列分析工具、CBS在线分析服务器软件、Conserved Domain Database(CDD)数据库、SABLE在线分析软件等对人类MEF2蛋白的不同亚型序列进行比较分析, 同时, 根据相关序列的比对结果构建系统进化树进行分析。结果表明, MEF2在人体内以多种蛋白形式存在, 其理化性质存在一定差别, 可能的翻译后糖基化修饰多为O型糖基化且均存在较多磷酸化位点。人类各MEF2蛋白具明显MADS结构域, 多数具有MEF2结构域和HJURP_C结构域。各MEF2蛋白二级结构均包括了螺旋、折叠和无规则卷曲等多种形式, 其三级结构模式相似。系统进化树显示MEF2B蛋白与其他蛋白有着较大的序列差异及较远进化关系, 可能较为原始。
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