综述

miRNA与siRNA胃癌相关研究的现状及进展

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  • 重庆医科大学附属第一医院普通外科, 重庆 400016

收稿日期: 2010-11-16

  修回日期: 2011-01-27

  网络出版日期: 2011-09-25

Current status and development of miRNA and siRNA research on gastric cancer

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  • Department of General Surgery, the First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China

Received date: 2010-11-16

  Revised date: 2011-01-27

  Online published: 2011-09-25

摘要

RNA干扰是表观遗传学的研究热点, 它参与基因复制后表达调控, 并与肿瘤发生密切相关。近年对RNA干扰研究较多的是微小RNA和小干扰RNA。文章概述了微小RNA和小干扰RNA的基本理论, 并综述它们在胃癌研究中的现状及进展。认为RNA干扰分析和应用是研究胃癌相关基因功能及作用机制的有效方法, 并将对胃癌的诊治产生巨大影响。

本文引用格式

何苗,王子卫 . miRNA与siRNA胃癌相关研究的现状及进展[J]. 遗传, 2011 , 33(9) : 925 -930 . DOI: 10.3724/SP.J.1005.2011.00925

Abstract

RNA interference (RNAi) is an important topic of epigenetics research in post-genome period. RNAi works as a post-DNA replication regulator for gene expression, and it is related to the occurrence and development of malignant tumors. The most usual participators of RNAi are MicroRNA (miRNA) and small interference RNA (siRNA). This review summarizes the basic theory of miRNA and siRNA, and provides recent progresses of RNAi research on gastric cancer. RNAi analysis and technique not only act as powerful tools for studying gene function and action mechanism, but also have diagnostic and therapeutic potential in gastric cancer, even in all kinds of tumors.

Key words: microRNA; siRNA; RNAi; gastric cancer

参考文献

[1] Lee Y, Ahn C, Han JJ, Choi H, Kim J, Yim J, Lee J, Provost P, Rådmark O, Kim S, Kim VN. The nuclear RNase III Drosha initiates microRNA processing. Nature, 2003, 425(6956): 415-419.
[2] Bartel DP. MicroRNAs: genomics, biogenesis, mechanism, and function. Cell, 2004, 116(2): 281-297.
[3] Garzon R, Liu SJ, Fabbri M, Liu ZF, Heaphy CEA, Callegari E, Schwind S, Pang JX, Yu JH, Muthusamy N, Havelange V, Volinia S, Blum W, Rush LJ, Perrotti D, Andreeff M, Bloom-field CD, Byrd JC, Chan K, Wu LC, Croce CM, Marcucci G. MicroRNA-29b induces global DNA hypomethylation and tumor suppressor gene reexpression in acute myeloid leukemia by targeting directly DNMT3A and 3B and indirectly DNMT1. Blood, 2009, 113(25): 6411-6418.
[4] Lewis BP, Burge CB, Bartel DP. Conserved seed pairing, often flanked by adenosines, indicates that thousands of human genes are microRNA targets. Cell, 2005, 120(1): 15-20.
[5] de Fougerolles A, Vornlocher HP, Maraganore J, Lieberman J. Interfering with disease: a progress report on siRNA-based therapeutics. Nat Rev Drug Discov, 2007, 6(6): 443-453.
[6] Stevenson M. Therapeutic potential of RNA interference. N Engl J Med, 2004, 351(17): 1772-1777.
[7] Kawasaki H, Taira K. Induction of DNA methylation and gene silencing by short interfering RNAs in human cells. Nature, 2004, 431(7005): 211-217.
[8] Yekta S, Shih IH, Bartel DP. MicroRNA-directed cleavage of HOXB8 mRNA. Science, 2004, 304(5670): 594-596.
[9] Song G, Zeng HZ, Li J, Xiao LF, He YZ, Tang YH, Li YR. miR-199a regulates the tumor suppressor mitogen-activated protein kinase kinase kinase 11 in gastric cancer. Biol Pharm Bull, 2010, 33(11): 1822-1827.
[10] Zhang ZY, Li ZJ, Gao CP, Chen P, Chen JJ, Liu WZ, Xiao SD, Lu H. miR-21 plays a pivotal role in gastric cancer pathogenesis and progression. Lab Invest, 2008, 88(12): 1358-1366.
[11] Tsujiura M, Ichikawa D, Komatsu S, Shiozaki A, Takeshita H, Kosuga T, Konishi H, Morimura R, Deguchi K, Fujiwara H, Okamoto K, Otsuji E. Circulating microRNAs in plasma of pa-tients with gastric cancers. Br J Cancer, 2010, 102(7): 1174-1179.
[12] Sun QM, Gu HJ, Zeng Y, Xia Y, Wang Y, Jing Y, Yang L, Wang B. Hsa-mir-27a genetic variant contributes to gastric cancer suscep-tibility through affecting miR-27a and target gene expression. Cancer Sci, 2010, 101(10): 2241-2247.
[13] Tsai KW, Hu LY, Wu CW, Li SC, Lai CH, Kao HW, Fang WL, Lin WC. Epigenetic regulation of miR-196b expression in gastric cancer. Genes Chromosomes Cancer, 2010, 49(11): 969-980.
[14] Feng RH, Chen XH, Yu YY, Su LP, Yu BQ, Li JF, Cai Q, Yan M, Liu BY, Zhu ZG. miR-126 functions as a tumour suppressor in human gastric cancer. Cancer Lett, 2010, 298(1): 50-63.
[15] Shinozaki A, Sakatani T, Ushiku T, Hino rR, Isogai M, Ishikawa S, Uozaki H, Takada K, Fukayama M. Downregulation of microRNA-200 in EBV-associated gastric carcinoma. Cancer Res, 2010, 70(11): 4719-4727.
[16] Tsukamoto Y, Nakada C, Noguchi T, Tanigawa M, Nguyen LT, Uchida T, Hijiya N, Matsuura K, Fujioka T, Seto M, Moriyama M. MicroRNA-375 is downregulated in gastric carcinomas and regulates cell survival by targeting PDK1 and 14-3-3ζ. Cancer Res, 2010, 70(6): 2339-2349.
[17] Ding L, Xu YJ, Zhang W, Deng YJ, Si MS, Du Y, Yao HM, Liu XY, Ke YH, Si JM, Zhou TH. MiR-375 frequently downregulated in gastric cancer inhibits cell proliferation by targeting JAK2. Cell Res, 2010, 20(7): 784-793.
[18] Wada R, Akiyama Y, Hashimoto Y, Fukamachi H, Yuasa Y. miR-212 is downregulated and suppresses methyl-CpG-binding protein MeCP2 in human gastric cancer. Int J Cancer, 2010, 127(5): 1106-1114.
[19] Suzuki H, Yamamoto E, Nojima M, Kai M, Yamano HO, Yoshi-kawa K, Kimura T, Kudo T, Harada E, Sugai T, Takamaru H, Ni-inuma T, Maruyama R, Yamamoto H, Toki
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