噬菌体展示技术(Phage display technology, PDT)是一种特殊的基因工程重组表达技术, 噬菌体展示技术系统(Phage display system, PDS)是指包括经过遗传改造后的系列噬菌体、辅助噬菌体、宿主细菌等集成平台(含试剂盒)。文章从噬菌体分子遗传学及其基因(基因组)遗传工程改良角度, 基于噬菌体M13、λ、T4和T7等4大类典型噬菌体展示技术系统的发展进展进行了综述。重点强调不同展示系统中的核心部件及其基因工程改造的分子遗传学原理、不同展示锚定位点的技术特征、相关试剂盒的研制状况及选择依据。
The phage display technology (PDT) was unique in genetic engineering and recombinant expression. The phage display systems (PDS) were platforms (kits) composed of genetic modified phages, helper phages, and host bacteria. This review concisely summarized the development of four types of PDS, based on M13, λ, T4, and T7 phages, in terms of phage molecular genetics and genetic (gene or genome) engineering. We addressed on the key components and their genetic (genomic) engineering for modifications, the technical features of different anchors, and the development progress and se-lection reference of those different kits.
[1] Smith GP. Filamentous fusion phage: novel expression vectors that display cloned antigens on the virion surface. Science, 1985, 228(4705): 1315-1317.
[2] MK 沃尔德, DI 弗里德曼, SL 阿迪亚主编, 艾云灿, 孟繁梅译. 噬菌体——在细菌致病机理及生物技术中的作用. 北京: 科学出版社, 2007: 409-422.
[3] Pande J, Szewczyk MM, Grover AK. Phage display: Concept, innovations, applications and future. Biotechnol Adv, 2010, 28(6): 849-858.
[4] Zani ML, Moreau T. Phage display as a powerful tool to engineer protease inhibitors. Biochimie, 2010, 92(11): 1689-1704.
[5] Maruyama IN, Maruyama HI, Brenner S. Lambda foo: a lambda phage vector for the expression of foreign proteins. Proc Natl Acad Sci USA, 1994, 91(17): 8273-8277.
[6] Ren ZJ, Black LW, Lewis GK, Wingfield PT, Locke EG, Steven AC, Black LW. Phage display of intact domains at high copy number: a system based on SOC, the small outer capsid protein of bacteriophage T4. Protein Sci, 1996, 5(9): 1833-1843.
[7] Rosenberg A, Griffin K, Studier FW, Mccormick M, Berg J, Novy R, Mierendorf R. T7 Select® Phage Display System: A powerful new protein display system based on bacteriophage T7. Innovations, 1996, (6): 1-6.
[8] Riechmann L, Holliger P. The C-terminal domain of TolA is the coreceptor for filamentous phage infection of E. coli. Cell, 1997, 90(2): 351-360.
[9] Devlin JJ, Panganiban LC, Devlin PE. Random peptide libraries: a source of specific protein binding molecules. Science, 1990, 249(4967): 404-406.
[10] Barbas CR 3rd, Kang AS, Lerner RA, Benkovic SJ. Assembly of combinatorial antibody libraries on phage surfaces: the gene III site. Proc Natl Acad Sci USA, 1991, 88(18): 7978-7982.
[11] Russel M. Moving through the membrane with filamentous phages. Trends Microbiol, 1995, 3(6): 223-228.
[12] Paschke M. Phage display systems and their applications. Appl Microbiol Biotechnol, 2006, 70(1): 2-11.
[13] Cwirla SE, Peters EA, Barrett RW, Dower WJ. Peptides on phage: a vast library of peptides for identifying ligands. Proc Natl Acad Sci USA, 1990, 87(16): 6378-6382.
[14] Fuh G, Sidhu SS. Efficient phage display of polypeptides fused to the carboxy-terminus of the M13 gene-3 minor coat protein. FEBS Lett, 2000, 480(2-3): 231-234.
[15] Sidhu SS, Weiss GA, Wells JA. High copy display of large proteins on phage for functional selections. J Mol Biol, 2000, 296(2): 487-495.
[16] Weiss GA, Sidhu SS. Design and evolution of artificial M13 coat proteins. J Mol Biol, 2000, 300(1): 213-219.
[17] Fuh G, Pisabarro MT, Li Y, Quan C, Lasky LA, Sidhu SS. Analysis of PDZ domain-ligand interactions using car-boxyl-terminal phage display. J Biol Chem, 2000, 275(28): 21486-21491.
[18] Wang KC, Wang XW, Zhong PY, Luo PP. Adapter-directed display: a modular design for shuttling display on phage surfaces. J Mol Biol, 2010, 395(5): 1088-1101.
[19] Gao CS, Mao SL, Lo CHL, Wirsching P, Lerner RA, Janda KD. Making artificial antibodies: a format for phage display of combinatorial heterodimeric arrays. Proc Natl Acad Sci USA, 1999, 96(11): 6025-6030.
[20] Hufton SE, Moerkerk PT, Meulemans EV, de Bruïne A, Arends JW, Hoogenboom HR. Phage display of cDNA repertoires: the pVI display system and its applications for the selection of immunogenic ligands. J Immunol Methods, 1999, 231(1-2): 39-51.
[21] Yang F, Forrer P, Dauter Z, Conway JF, Cheng N, Cerritelli ME, Steven AC, Plückthun A, Wlodawer A. Novel fold and capsid-binding properties of the lambda-phage display platform protein gpD. Nat Struct Biol, 2000, 7(3): 230-237.
[22] Mikawa YG, Maruyama IN, Brenner S. Surface display of proteins on bacteriophage λ heads. J Mol Biol, 1996, 262(1): 21-30.
[23] Sternberg N, Hoess RH. Display of peptides and proteins on the surface of bacteriophage lambda. Pro
