近年来, 来自农杆菌的致瘤基因在植物中的功能和作用机理研究越来越得到重视。6b基因是植物肿瘤形成的重要影响因子, 它位于T-DNA片段上, 属于rolB基因家族。在植物体内, 6b基因能影响激素水平、糖的含量和其他次生代谢产物的积累, 以及改变与其相关基因的表达水平等, 但其具体作用机制还有待深入研究。文章对6b基因的生物功能、分子结构、活性部位、作用模式等方面的重要研究进行了综述, 为这类功能基因的进一步研究及应用提供了理论依据。
In recent years, the functional mechanisms of the oncogenens from Agrobacterium in plants were received more and more attentions. 6b genes, derived from the T-DNA fragment, are vital carcinogenesis factors of plants and belong to rolB genes family. In plants, 6b genes can affect phytohormone levels and carbohydrate contents, and can also cause accumulation of secondary metabolites, as well as change the relative genes expression. The specific mechanisms behind these impacts remain to be researched in-depth. In this paper, the function, structure, activity, and acting mode of the 6b genes were summarized, which provide a theoretical foundation for further study and application of these functional genes.
[1] Zhu CF, Wu JH, He CZ. Induction of chromosomal inversion by integration of T-DNA in the rice genome. J Genet Genomics, 2010, 37(3): 189-196.
[2] Clément B, Pollmann S, Weiler E, Urbanczyk-Wochniak E, Otten L. The Agrobacterium vitis T-6b oncoprotein induces auxin-independent cell expansion in tobacco. Plant J, 2006, 45(6): 1017-1027.
[3] Gaudin V, Vrain T, Jouanin L. Bacterial genes modifying Hormonal balances in plants. Plant Physiol Bio-chem, 1994, 32(1): 11-29.
[4] Meyer A, Tempe J, Costantino P. Hairy root: a molecular overview. Functional analysis of Agrobacterium rhizogenes T-DNA genes. In: Stacey G, Keen NT, eds. Plant microbe interactions. Paul: APS Press, 2000: 93-139.
[5] Nilsson O, Olsson O. Getting to the root: the role of the Agrobacterium rhizogenes rol genes in the formation of hairy roots. Physiol Plant, 1997, 100(3): 463-473.
[6] Messens E, Lenaerts A, van Montagu M, Hedges RW. Genetic basis for opine secretion from crown gall tumour cells. Mol Gen Genet, 1985, 199(2): 344-348.
[7] Otten L, Helfer A. Biological activity of the rolB-like 5′end of the A4-orf8 gene from the Agrobacterium rhizogenes TL-DNA. Mol Plant Microbe Interact, 2001, 14(3): 405-411.
[8] Altamura MM, Capitani F, Gazza L, Capone I, Costantino P. The plant oncogene rolB stimulates the formation of flower and root meristemoids in tobacco thin cell layers. New Phytol, 1994, 126(2): 283-293.
[9] Capone I, Cardarelli M, Mariotti D, Pomponi M, De Paolis A, Costantino P. Different promoter regions control level and tissue specificity of expression of Agrobacterium rhizogenes rolB gene in plants. Plant Mol Biol, 1991, 16(3): 427-436.
[10] Schmulling T, Schell J, Spena A. Single genes from Agrobacterium rhizogenes influence plant development. EMBO J, 1988, 7(9): 2621-2629.
[11] Estruch JJ, Schell J, Spena A. The protein encoded by the rolB plant oncogene hydrolyses indole glucosides. EMBO J, 1991, 10(11): 3125-3128.
[12] Schröder G, Waffenschmidt S, Weiler EW, Schröder J. The T-region of Ti plasmids codes for an enzyme synthesizing indole-3-acetic acid. Eur J Biochem, 1984, 138(2): 387-391.
[13] Thomashow MF, Hugly S, Buchholz WG, Thomashow LS. Molecular basis for the auxin-independent phenotype of crown gall tumor tissues. Science, 1986, 231(4738): 616-618.
[14] Maurel C, Brevet J, Barbier-Brygoo H, Guern J, Tempé J. Auxin regulates the promoter of the root-inducing rolB gene of Agrobacterium rhizogenes in transgenic tobacco. Mol Gen Genet, 1990, 223(1): 58-64.
[15] Wabiko H, Minemura M. Exogenous phytohormone-independent growth and regeneration of tobacco plants transgenic for the 6b gene of Agrobacterium tumefaciens AKE10. Plant Physiol, 1996, 112(3): 939-951.
[16] Gális I, Šimek P, Macas J, Zahradní?ková H, Vlasák J, Wabiko H, van Dongen W, van Onckelen HA, Ond?ej M. The Agrobacterium tumefaciens C58-6b gene confers resistance to N6-benzyladenine without modifying cytokinin metabolism in tobacco seedlings. Planta, 1999, 209(4): 453-461.
[17] Helfer A, Pien H, Otten L. Functional diversity and mutational analysis of Agrobacterium 6b oncoproteins. Genet Genomics, 2002, 267(5): 577-586.
[18] Wang MM, Soyano T, Machida S, Yang JY, Jung C, Chua NH, Yuan YA. Molecular insights into plant cell proliferation disturbance by Agrobacterium protein 6b. Genes Dev, 2011, 25(1): 64-76.
[19] Helfer A, Clément B, Michler P, Otten L. The Agrobacterium oncogene AB-6b causes a graft-transmissible enation syndrome in tobacco. Plant Mol Biol, 2003, 52(2): 483- 493.
[20] Grémillon L, Helfer A, Cl