两系杂交小麦恢复系MR168抗条锈病基因遗传分析及分子标记定位

任勇，李生荣，李俊，周强，杜小英，李太军，杨武云，郑有良

doi:10.3724/SP.J.1005.2011.01263

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2011 , Vol. 33 >Issue 11: 1263 - 1270

DOI: https://doi.org/10.3724/SP.J.1005.2011.01263

研究报告

两系杂交小麦恢复系MR168抗条锈病基因遗传分析及分子标记定位

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1. 绵阳市农业科学研究院, 国家小麦改良中心绵阳分中心, 绵阳 621023 2. 四川省农业科学院作物研究所, 成都 610066 3. 四川农业大学小麦研究所, 成都 611130

收稿日期: 2011-02-15

修回日期: 2011-04-27

网络出版日期: 2011-11-25

基金资助

国家高技术研究发展计划项目(863计划)(编号：2009AA101102)和现代农业产业技术体系建设专项资金(编号：nycytx-03)资助

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Genetic analysis and molecular mapping of stripe rust resistance gene in a restore line of Thermo-Photo sensitive hybrid wheat MR168

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1. Mianyang Branch of National Wheat Improvement Center, Mianyang Academy of Agricultural Sciences, Mianyang 621023, China 2. Crop Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610066, China 3. Triticeae Research Institute, Sichuan Agricultural University, Chengdu 611130, China

Received date: 2011-02-15

Revised date: 2011-04-27

Online published: 2011-11-25

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摘要

小麦条锈病是影响杂交小麦普及推广的重要因素。文章利用基因推导法和SSR分子标记技术, 研究了温光型两系杂交小麦恢复系MR168的抗条锈性遗传规律及其控制基因染色体位置。结果表明, MR168对CY29、CY31、CY32、CY33等条锈菌生理小种表现高抗至免疫; 对SY95-71/MR168杂交组合的正反交F₁、BC₁、F₂和F₃群体分单株接种鉴定显示, MR168对CY32号小种的抗性受1对显性核基因控制, 该抗病基因来源于春小麦品种辽春10号。利用集群分离分析法(Bulked segregant analysis, BSA)和简单重复序列(Simple sequence repeat, SSR)分子标记分析抗病亲本MR168、感病亲本SY95-71及183个F₂代单株, 发现了与MR168抗条锈病基因连锁的5个微卫星标记Xgwm273、Xgwm18、Xbarc187、Xwmc269、Xwmc406, 并将该基因初步定位在1BS着丝粒附近, 暂命名为YrMR168; 构建了包含YrMR168的SSR标记遗传图谱, 距离YrMR168最近的两个微卫星位点是Xgwm18和Xbarc187, 遗传距离分别为1.9 cM和2.4 cM, 这两个微卫星标记可用于杂交小麦抗条锈病分子标记辅助育种。

关键词： 杂交小麦; 恢复系; 抗条锈病基因; 基因推导; 遗传分析; 微卫星标记

本文引用格式

任勇，李生荣，李俊，周强，杜小英，李太军，杨武云，郑有良 . 两系杂交小麦恢复系MR168抗条锈病基因遗传分析及分子标记定位[J]. 遗传, 2011 , 33(11) : 1263 -1270 . DOI: 10.3724/SP.J.1005.2011.01263

Abstract

Stripe rust, caused by Puccinia striiformis f. sp. tritici, is an important limiting factor to popularize hybrid wheat. The objectives of this study were to map a stripe rust resistance gene in a Chinese thermo-photo-sensitive hybrid wheat restore line MR168 using gene postulation and SSR markers. MR168 was highly resistant to 23 Pst races including CYR29, CYR31, CYR32, and CYR33. The populations F₁, BC₁, F₂, and F₃ from the cross between MR168 and SY95-71 (a wheat cultivar susceptible to Pst races) were inoculated with the race of Pst CYR32 of China in greenhouse. MR168 carried a single dominant gene for resistance to CYR32, tentatively designated YrMR168. It originated from Liaochun 10, a spring wheat variety. A total of 183 F₂ plants, the resistant and susceptible parents and resistant and susceptible bulks were used for resistance gene mapping with 329 pairs of wheat SSR markers.Five SSR markers on chromosome 1BS including Xgwm18, Xbarc187, Xwmc269, Xgwm273, and Xwmc406 were linked with YrMR168. The resistance gene was closely linked to Xgwm18 and Xbarc187 with the genetic distances of 1.9 and 2.4 cM, respectively. Xgwm18 and Xbarc187 could be used for molecular marker assisted selection of YrMR168 in hybrid wheat breeding program.

Key words： hybrid wheat; restore line; stripe rust; resistance gene; gene postulation; SSR marker

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