以重复序列pAS1和pSc119.2为探针, 对八倍体小黑麦×普通小麦的F5代植株进行了FISH分析, 同时对这些材料进行了田间抗病性鉴定。从中鉴定出了1R、2R、3R、4R、5R、6R、7R单体附加系和1R、2R二体附加系, 1R和4R附加系出现频率相对较高。5R和6R单体附加系对白粉病免疫, 推测5R染色体上带有新的白粉病抗性基因。此外, 还检测到不少植株染色体组发生了变异, 且小麦4B染色体优先缺失。
The F5 plants derived from octoploid triticale × common wheat were investigated by FISH methods using repetitive DNA sequences pAS1 and pSc119.2 as probes. The disease resistance of these plants was also screened and evaluated in the field. The 1R, 2R, 3R, 4R, 5R, 6R and 7R monosomic addition lines and 1R and 2R disomic addition lines were found. The occurrence frequencies of chromosomes 1R and 4R addition lines were higher than that of chromosomes 2R, 3R, 5R, 6R, and 7R addition lines in the high generation screened. The 5R and 6R monosomic addition lines were im-mune to powdery mildew. The chromosome 5R in this study might carry new powdery mildew resistance gene(s). In addi-tion, the preferential elimination of chromosome 4B was observed in several plants.
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