应用引物复性控制技术筛选肌内脂肪含量差异极显著的延边黄牛背最长肌组织差异表达基因, 寻找与肌内脂肪沉积的相关候选基因。文章选取30头28月龄延边黄牛阉牛的背最长肌组织测定肌内脂肪含量, 选取肌内脂肪含量差异极显著的最高和最低各3头组成RNA池, 采用引物复性控制技术, 分析了两组个体背最长肌组织差异表达基因。利用20对随机引物差异显示扩增下, 共获得12条ESTs(片段大小为200～890 bp), 其中8个为已知的ESTs分别与细胞骨架形成、细胞因子信号转导、蛋白质合成、能量代谢和其他功能的差异基因, 4个未知的ESTs。结果表明, 应用引物复性控制技术筛选得到了12个可能参与了肌内脂肪沉积调控的ESTs, 为进一步筛选肌内脂肪沉积相关的基因奠定了基础。
Annealing control primer (ACP) system was applied to find candidate genes related to lipidosis in muscle of Yanbian yellow cattle by screening differentially expressed genes (DEGs) in Longissimus dorsi, which had significant dif-ference on intramuscular fat (IMF) content. Thirty steers, aged at 28 month-bullocks were selected to measure the IMF content in L. dorsi. Two groups of bullocks (three heads per group) with the highest and the lowest contents of IMF were selected to build a RNA pool, and DEGs of two groups were analyzed by ACP system. Twelve DEGs were identified and sequenced by amplification with 20 arbitrary primers (fragment sizes were 200–890 bp). In these genes, eight were already known as functional groups of cytoskeleton, cytokine signal transduction, protein synthesis, energy metabolismand, and others, four were unknown. All the 12 ESTs were screened by ACP system, which may participated in regulating on lipidosis in muscle. This study established a foundation for further screening of lipidosis related genes.
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