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新一代测序技术在植物转录组研究中的应用

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  • 中国科学院植物研究所资源植物研发重点实验室, 北京 100093

收稿日期: 2010-11-16

  修回日期: 2011-08-24

  网络出版日期: 2011-12-25

基金资助

国家重点基础研究发展计划项目(“973”计划)(编号:2007CB108905)资助

Application of next generation sequencing techniques in plant transcriptome

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  • The Research and Development Laboratory for Plant Resources, Institute of Bontany, Chinese Acdemy of Sciences, Beijing 100093, China

Received date: 2010-11-16

  Revised date: 2011-08-24

  Online published: 2011-12-25

摘要

随着DNA测序技术的发展, 新一代测序技术以其高通量、低成本的特点, 成为越来越多的生物学研究者在开展工作时的首选。在所有的新一代测序技术中, 454测序系统是最早实现商业化且发展相对成熟的一种, 目前被广泛的应用于各个领域的生物学研究中。文章以454测序系统为例, 综述了新一代测序系统的原理、优缺点, 及其在植物转录组研究中的应用, 并对其在植物研究领域中可能的发展应用方向进行了展望。

本文引用格式

梁烨,陈双燕,刘公社 . 新一代测序技术在植物转录组研究中的应用[J]. 遗传, 2011 , 33(12) : 1317 -1326 . DOI: 10.3724/SP.J.1005.2011.01317

Abstract

With the development of DNA sequencing techniques, the next-generation sequencing (NGS) techniques with the characteristics of high-throughput and low cost have become the first choice for more and more researchers to carry out the biological researches. Among the next-generation sequencing techniques, the 454 sequencing platform is the first com-mercially available and relatively mature one and widely used in various fields of biological research. Taking 454 sequenc-ing platform as an example, we illustrate the advantages and disadvantages of NGS technical principles, review their appli-cations in plant transcriptome, and outlook their future development and applications in plant research field.

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