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miRNA*生物合成及其功能研究的新发现

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  • 浙江大学生命科学院遗传所, 杭州 310058

收稿日期: 2011-11-11

  修回日期: 2012-01-05

  网络出版日期: 2012-04-25

基金资助

国家自然科学基金项目(编号:30972016, 31171615, 31171543)和浙江省自然科学基金(编号:Y3090247)资助

Recent research progress of biogenesis and functions of miRNA

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  • Institution of Genetics, College of Life Science, Zhejiang University, Hangzhou 310058, China

Received date: 2011-11-11

  Revised date: 2012-01-05

  Online published: 2012-04-25

摘要

miRNA*是在miRNA加工成熟过程中与其互补的大约22个核苷酸的RNA序列。传统观点认为miRNA*是miRNA生物合成中形成的没有功能的副产品。然而最近的研究发现miRNA*s与miRNA一样, 主要介导转录后的基因调控网络; 但不同的是, miRNA与Argonaute1蛋白(AGO1)结合形成RNA诱导的沉默复合体(RISC), 而miRNA*却在AGO2的帮助下形成RISC复合体进行RNA干涉, 这点与siRNA的作用方式类似。文章从miRNA*的生物合成、生物学特性和功能等方面综述了miRNA*最新研究进展。

本文引用格式

马圣运,白玉,韩凝,王君晖,翁晓燕,边红武,朱睦元 . miRNA*生物合成及其功能研究的新发现[J]. 遗传, 2012 , 34(4) : 383 -388 . DOI: 10.3724/SP.J.1005.2012.00383

Abstract

MicroRNA*s are about 22nt noncoding RNAs, which are processed from precursors with a characteristic hairpin secondary structure in the biogenesis of microRNAs. Recently, miRNA* strands were shown to mediate post-transcriptional regulatory networks, rather than serve merely as non-functional by-product in general view. Unlike miRNAs bound to AGO1, miRNA* strands are bound to AGO2 to form RISC duplex to mediate RNAi, which is similar to siRNA. This paper mainly reviewed the recent research progresses on miRNA*, such as the biosynthesis, biological characteristics, and functions.

Key words: miRNA*; miRNA; RNAi; AGO; miR/miR* duplex

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