综述

细胞外微RNA:一种新型的肺癌分子生物标志物

展开
  • 宁波大学医学院生物化学与分子生物学研究所, 宁波315211

收稿日期: 2012-01-12

  修回日期: 2012-03-09

  网络出版日期: 2012-06-25

基金资助

浙江省自然科学基金项目(编号:Y12C060009), 浙江省教育厅重点项目(编号:Z201119414), 宁波市科技创新团队项目(2011B82014)和宁波大学王宽诚教育基金项目资助

Extracellular miRNA: a novel molecular biomarker for lung cancer

Expand
  • Institute of Biochemistry and Molecular Biology, School of Medicine, Ningbo University, Ningbo 315211, China

Received date: 2012-01-12

  Revised date: 2012-03-09

  Online published: 2012-06-25

摘要

尽管癌症的早期诊断和治疗在不断发展和进步, 但寻找一种敏感、准确和微创的分子生物标志物, 用于肿瘤的诊断仍是一项艰巨任务。微RNA(miRNA)是一类长约21~24个核甘酸的内源性非编码小分子RNA。细胞外miRNA作为一种新型的分子生物标志物, 在癌症诊断方面具有微创、高灵敏度和高特异性等许多潜在特征。近年来, 细胞外miRNA研究成果颇丰。文章就细胞外miRNA的来源、功能、检测以及作为分子标志物在肺癌诊断中的作用和目前存在的一些问题进行了综述。

本文引用格式

杨丽华,董琢,龚朝辉 . 细胞外微RNA:一种新型的肺癌分子生物标志物[J]. 遗传, 2012 , 34(6) : 651 -658 . DOI: 10.3724/SP.J.1005.2012.00651

Abstract

Though continuous development and progress have been made in the early diagnosis and treatment of cancer, it is still difficult to find a sensitive, accurate and minimally invasive biomarker for cancer diagnosis and treatment. MicroRNA (miRNA) is a class of non-coding small endogenous RNAs of 21-24 nucleotides in length. As a novel molecular biomarker, extracellular miRNA (ec-miRNA) has the potential to be a minimally invasive, highly sensitive and highly specific marker in cancer diagnosis. Many research achievements of ec-miRNA have been accumulated in recent years. In this paper, the origin, function and detection of ec-miRNA, its role in lung cancer diagnosis as a novel molecular biomarker, and some issues are reviewed.

参考文献

[1] American Cancer Society. Cancer Facts & Figures 2012. Atlanta: American Cancer Society, 2012.
[2] Maddalena B, Armando F, Massimo R, Salvatore G, Walter M, Malorni GP. Reducing the risk of overdiagnosis in lung cancer: A support from molecular biology. Cell Physiol, 2011, 226(9): 2213-2214.
[3] Hu Z, Chen X, Zhao Y, Tian T, Jin G, Shu Y, Chen Y, Xu L, Zen K, Zhang C, Shen H. Serum microRNA signatures identified in a genome-wide serum microRNA expression profiling predict survival of non-small-cell lung cancer. J Clin Oncol, 2010, 28(10): 1721-1726.
[4] Ferracin M, Veronese A, Negrini M. Micromarkers: miRNAs in cancer diagnosis and prognosis. Expert Rev Mol Diagn, 2010, 10(3): 297-308.
[5] Cortez MA, Calin GA. MicroRNA identification in plasma and serum: a new tool to diagnose and monitor diseases. Expert Opin Biol Ther, 2009, 9(6): 703-711.
[6] Wang QZ, Xu W, Habib N, Xu R. Potential uses of mi-croRNA in lung cancer diagnosis, prognosis, and therapy. Curr Cancer Drug Targets, 2009, 9(4): 572-594.
[7] Weber JA, Baxter DH, Zhang S, Huang DY, Huang KH, Lee MJ, Galas DJ, Wang K. The microRNA spectrum in 12 body fluids. Clin Chem, 2010, 56(11): 1733-1741.
[8] Zubakov D, Boersma AWM, Choi Y, van Kuijk PF, Wiemer EAC, Kayser M. MicroRNA markers for forensic body fluid identification obtained from microarray screening and quantitative RT-PCR confirmation. Int J Legal Med, 2010, 124(3): 217-226.
[9] Hanson EK, Lubenow H, Ballantyne J. Identification of forensically relevant body fluids using a panel of differentially expressed microRNAs. Anal Biochem, 2009, 387(2): 303-314.
[10] Arroyo JD, Chevillet JR, Kroh EM, Ruf IK, Pritchard CC, Gibson DF, Mitchell PS, Bennett CF, Pogosova-Agadjanyan EL, Stirewalt DL, Tait JF, Tewari M. Argonaute2 complexes carry a population of circulating microRNAs inde-pendent of vesicles in human plasma. Proc Natl Acad Sci USA, 2011, 108(12): 5003-5008.
[11] Hunter MP, Ismail N, Zhang XL, Aguda BD, Lee EJ, Yu LL, Xiao T, Schafer J, Lee ML, Schmittgen TD, Nana- Sinkam SP, Jarijoura D, Marsh CB. Detection of microRNA expression in human peripheral blood microvesi-cles. PLoS One, 2008, 3(11): e3694.
[12] Hadi V, Karin E, Apostolos B, Margareta S, James JL, Jan OL. Exosome-mediated transfer of mRNAs and microR-NAs is a novel mechanism of genetic exchange between cells. Nat Cell Biol, 2007, 9(6): 654-659.
[13] Taylor DD, Gercel-Taylor C. MicroRNA signatures of tumor-derived exosomes as diagnostic biomarkers of ovarian cancer. Gynecol Oncol, 2008, 110(1): 13-21.
[14] Zheng D, Haddadin S, Wang Y, Gu LQ, Perry MC, Freter CE, Wang MX. Plasma microRNAs as novel biomarkers for early detection of lung cancer. Int J Clin Exp Pathol, 2011, 4(6): 575-586.
[15] Gibbings DJ, Ciaudo C, Erhardt M, Voinnet O. Multive-sicular bodies associate with components of miRNA effector complexes and modulate miRNA activity. Nat Cell Biol, 2009, 11(9): 1143-1149.
[16] Etheridge A, Lee I, Hood L, Galas D, Wang K. Extracel-lular microRNA: A new source of biomarkers. Mutat Res, 2011, 717(1-2): 85-90.v
[17] Mitchell PS, Parkin RK, Kroh EM, Fritz BR, Wyman SK, Pogosova-Agadjanyan EL, Peterson A, Noteboom J, O'Briant KC, Allen A, Lin DW, Urban N, Drescher CW, Knudsen BS, Stirewalt DL, Gentleman R, Vessella RL, Nelson PS, Martin DB, Tewari M. Circulating microRNAs as stable blood-based markers for cancer detection. Proc Natl Acad Sci USA, 2008, 105(30): 10513-10518.
[18] Park NJ, Zhou H, Elashoff D, Henson BS, Kastratovic DA, Abemayor E, Wong DT. Salivary microRNA: discovery, characterization, and clinical utility for oral cancer detection. Clin Cancer Res, 2009, 15(17): 5473-5477.
[19] Turchinovich A, Weiz L, Langheinz A, Burwinkel B. Characterization of extracellular circulating microRNA. Nucleic Acids Res, 2
文章导航

/