将染色质免疫共沉淀技术(ChIP)与下一代高通量测序技术相结合的染色质免疫共沉淀测序(ChIP-seq), 已成为功能基因组学、特别是基因表达调控领域研究的关键技术。ChIP-seq实验带来的海量数据向生物信息学研究人员提出了新的挑战。由于此领域数据处理技术的发展大大滞后于实验技术进步, 有必要系统地介绍和回顾ChIP-seq数据处理的各个方面, 以便更多研究人员进入此领域设计或改进相应的算法。文章结合实例详细介绍了ChIP-seq数据整个流程, 并重点讨论了其中的主要问题和关键环节, 为这一研究领域的科研人员提供一个快速而深入的认识。
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