幽门螺杆菌<em>vacA</em>和<em>cagA</em>基因全长分子系统发育分析

杨泽民，陈蔚文

doi:10.3724/SP.J.1005.2012.00863

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2012 , Vol. 34 >Issue 7: 863 - 871

DOI: https://doi.org/10.3724/SP.J.1005.2012.00863

研究报告

幽门螺杆菌vacA和cagA基因全长分子系统发育分析

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1. 广东药学院基础学院, 广州 510006 2. 广州中医药大学脾胃研究所, 广州 510405 3. 上海市高校中医内科学E-研究院, 上海中医药大学, 上海 201203

收稿日期: 2011-11-21

修回日期: 2011-12-27

网络出版日期: 2012-07-25

基金资助

国家自然科学基金项目(编号:81102703), 国家自然科学基金重大研究计划重点资助项目(编号：90209004), 广东省自然科学基金重点资助项目(编号：05102323)和上海市教育委员会E-研究院建设计划资助项目(编号：E03008)资助

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Molecular phylogeny analysis of full-length vacA and cagA genes from Helicobacter pylori

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1. School of Basic Courses, Guangdong Pharmaceutical University, Guangzhou 510006, China 2. Pi-Wei Institute, Guangzhou University of Traditional Chinese Medicine, Guangzhou 510405, China 3. E-Institute of Shanghai Municipal Education Commission, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China

Received date: 2011-11-21

Revised date: 2011-12-27

Online published: 2012-07-25

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摘要

文章从GenBank中下载所有含有vacA和cagA基因的H. pylori菌株的VacA和CagA全长氨基酸序列, 利用ClastalX 2.0和MEGA 5.05软件构建VacA和CagA分子系统发育树, 探讨两基因之间的分子系统发育关系和不同聚类群的临床感染结果与基因型特征。结果显示, VacA和CagA具有高度相似的分子系统发育树, 并且所有H. pylori菌株在系统发育树中具有相同的分布特点, 分别聚类为东亚株群1、2和西方株群3个聚类群。其中东亚株群1患萎缩性胃炎比例较高, vacA基因型以s1c/m1b和s1a/m1b为主, cagA基因型以EPIYA-ABD为主; 东亚株群2患十二指肠溃疡的比例较高, vacA基因型以s1c/m2和s1a/m2为主, cagA基因型以EPIYA-AB'C为主; 西方株群患十二指肠溃疡和胃炎的比例相当, 萎缩性胃炎比例较低, vacA基因型以s1a/m1a和s1b/m1a为主, cagA基因型以EPIYA-AB/B'CC为主。这些结果说明, vacA和cagA基因可能具有共进化的遗传关系; 东亚株群1、2和西方株群分别具有不同的vacA和cagA基因亚型, 这可能与其临床感染结果密切相关, 因此, 在进行H. pylori相关性疾病分析时, 有必要结合vacA和cagA基因型的亚型做深入分析。

关键词： 幽门螺杆菌; vacA; cagA; 系统发育树; 基因型; 临床结果

本文引用格式

杨泽民，陈蔚文 . 幽门螺杆菌vacA和cagA基因全长分子系统发育分析[J]. 遗传, 2012 , 34(7) : 863 -871 . DOI: 10.3724/SP.J.1005.2012.00863

Abstract

All amino acid full-length sequences of VacA and CagA proteins from Helicobacter pylori strains including vacA and cagA genes were downloaded from GenBank. Molecular phylogenic trees of VacA and CagA were constructed by ClastalX 2.0 and MEGA 5.05 software to understand phylogenetic relationships of vacA and cagA genes, clinical infection effects, and genotype characteristics of different clustering groups. The results showed that the phylogenetic trees of VacA and CagA recapitulated the same three-clustering groups, i.e., East Asia group 1 and 2 and Western group, and all H. pylori strains had similar distribution. The strains of East Asia group 1 were significantly higher in patients with atrophic gastritis. Genotype vacA contained mainly s1c/m1b and s1a/m1b, while genotype cagA was mostly EPIYA-ABD. The strains of East Asia group 2 were higher in patients with duo-denal ulcer. Genotype vacA was mainly s1c/m2 and s1a/m2, while genotype cagA was mostly EPIYA-AB'C. The strains of Western group were higher in patients with duodenal ulcer and chronic gastritis than with atrophic gastritis. Genotype vacA was mainly s1a/m1a and s1b/m1a, while genotype cagA was mostly EPIYA-AB/B'CC. All of these results illustrated that there might be inheritant relationship of coevolution between vacA and cagA genes; East Asia group 1 and 2 and Western group had different vacA and cagA sub-genotypes, which had close relationship to its clinical infection effects. It might be necessary to deeply analyze vacA and cagA sub-genotypes in the research of H. pylori-related diseases.

Key words： Helicobacter pylori; vacA; cagA; phylogenetic tree; gene type; clinical effect

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