为探讨鸡视网膜母细胞瘤基因1(Retinoblastoma1, RB1)多态性对体重性状的影响, 文章以东北农业大学高、低脂双向选择品系肉鸡为实验材料, 采用MALDI-TOF-MS、PCR-RFLP方法进行基因多态性检测和个体基因型分析, 共获得27个SNP位点的基因型数据。采用滑动窗口法构建单倍型, 进而利用单位点和单倍型分别与鸡体重性状进行关联分析。结合单位点和单倍型分析结果, 确定了RB1基因上4个显著影响1周龄体重的SNP位点, 2个显著影响1、3周龄体重的SNP位点。研究结果表明RB1基因是影响鸡早期体重性状的重要候选基因。
The Northeast Agricultural University broiler lines divergently selected for abdominal fat content (NEAUHLF) were used in the current study to investigate the effects of Retinoblastoma1 (RB1) gene on chicken body weight (BW). The single nucleotide polymorphisms (SNPs) of the individuals were detected by MALDI-TOF-MS and PCR-RFLP methods and the genotypes of 27 SNPs were obtained. Haplotypes were constructed by liding window approach. Association analysis between single SNPs and haplotypes and body weight were conducted, respectively. Based on the results of single SNPs and haplotype association analyses. Four SNPs with significant effects on body weight at 1 week of age (BW1) and 2 SNPs with significant effects on BW1 and BW3 were determined. These results suggested that RB1 is an important candidate gene that affects chicken early growth and development.
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