研究报告

籼稻稻米碾磨与外观品质性状的QTL定位

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  • 中国水稻研究所, 国家水稻改良中心/水稻生物学国家重点实验室, 杭州 310006

收稿日期: 2012-03-03

  修回日期: 2012-06-19

  网络出版日期: 2012-12-25

基金资助

国家高技术研究发展规划项目(863计划)(编号:2011AA10A101); 国家转基因生物新品种培育重大专项(编号:2011ZX08001-004)和中央级公益性科研院所基本科研业务费专项(编号:2009RG002)资助

Mapping QTL for rice milling and appearance quality traits in indica rice

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  • Chinese National Center for Rice Improvement/State Key Laboratory of Rice Biology, China National Rice Research Institute, Hang-zhou 310006, China

Received date: 2012-03-03

  Revised date: 2012-06-19

  Online published: 2012-12-25

摘要

文章利用籼籼交组合特青/IRBB衍生的重组自交系群体, 在2个环境下对稻米碾磨品质和外观品质进行QTL定位。共计检测到控制稻米碾磨品质的QTL 12个和控制外观品质的QTL 18个, 包括糙米率8个、精米率2个、整精米率2个、粒长7个、粒宽5个和长宽比6个, 这些QTL分布于除第4和12染色体外的其他10条染色体上。其中, 第3染色体涵盖粒形基因GS3的区域对粒长、长宽比、糙米率和整精米率具有较大效应, 其献率分别为56.71%、42.23%、10.05%和4.91%; 第5染色体涵盖粒宽基因GW5的区域对粒宽、长宽比、糙米率和精米率具有较大效应, 表型变异贡献率分别为59.51%、36.68%、19.51%和4.56%。此外, 第6染色体涵盖直链淀粉含量基因Wx的区域对糙米率和精米率具有较小效应。GS3和GW5对糙米率和粒形具有重要作用。

本文引用格式

梅德勇,朱玉君,樊叶杨 . 籼稻稻米碾磨与外观品质性状的QTL定位[J]. 遗传, 2012 , 34(12) : 1591 -1598 . DOI: 10.3724/SP.J.1005.2012.01591

Abstract

Quantitative trait loci (QTL) controlling six milling and appearance quality traits were analyzed over 2 years using recombinant inbred lines derived from two indica rice Teqing and IRBB. A total of 30 QTL for these traits were detected, of which eight were for brown rice rate (BRR), two for milled rice recovery (MRR), two for head rice recovery (HRR), seven for grain length (GL), five for grain width (GW), and six for length/width ratio (LWR). The QTL were dis-tributed on all chromosomes except for chromosomes 4 and 12. A QTL cluster with major effects on GL, LWR, BRR, and HRR was located in the RM15139-RM15303 interval on chromosome 3, which includes the GS3 gene for grain size. The phenotypic variances explained by the QTL were 59.51%, 36.68%, 19.51%, and 4.56%, respectively. QTL affecting GW, LWR, BRR, and MRR were clustered in the RM437-RM18038 region of chromosome 5,which covers the GW5 gene for grain width, and contributed 59.51%, 36.68%, 19.51%, and 4.56% to the total variance. QTL with minor effects on BRR and MRR were mapped to the RM190-RM587 interval covering the Wx gene for amylase content on chromosome 6. These results suggest that GS3 and GW5 may play a major roles in the genetic control of BRR and grain shape.

参考文献

[1] 周立军, 江铃, 翟虎渠, 万建民. 水稻垩白的研究现状与改良策略. 遗传, 2009, 31(6): 563-572.
[2] 张玉华. 稻米的碾磨品质及其影响因素. 中国农学通报, 2003, 19(1): 101, 158.
[3] 石春海, 朱军. 水稻植株农艺性状与稻米碾磨品质的遗传相关性分析. 浙江大学学报(农业与生命科学版), 1997, 23(3): 331-337.
[4] 徐正进, 陈温福, 马殿荣, 吕英娜, 周淑清, 刘丽霞. 稻谷粒形与稻米主要品质性状的关系. 作物学报, 2004, 30(9): 894-900.
[5] 王丹英, 章秀福, 朱智伟, 陈能, 闵捷, 姚青, 严建立, 廖西元. 食用稻米品质性状间的相关性分析. 作物学报, 2005, 31(8): 1086-1091.
[6] Tan YF, Xing YZ, Li JX, Yu SB, Xu CG, Zhang QF. Ge-netic bases of appearance quality of rice grains in Shanyou 63, an elite rice hybrid. Theor Appl Genet, 2000, 101(5-6): 823-829.
[7] Tan YF, Sun M, Xing YZ, Hua JP, Sun XL, Zhang QF, Corke H. Mapping quantitative trait loci for milling qual-ity, protein content and color characteristics of rice using a recombinant inbred line population derived from an elite rice hybrid. Theor Appl Genet, 2001, 103(6-7): 1037- 1045.
[8] Kepiro JL, McClung AM, Chen MH, Yeater KM, Fjell-strom RG. Mapping QTLs for milling yield and grain characteristics in a tropical japonica long grain cross. J Cereal Sci, 2008, 48(2): 477-485.
[9] 穆平, 郭咏梅, 刘家富, 卢义宣, 李自超. 稻米外观和碾磨品质QTL定位及其与土壤水分环境互作分析. 农业生物技术学报, 2007, 15(4): 654-660.
[10] Dong YJ, Tsuzuki E, Lin DZ, Kamiunten H, Terao H, Matsuo M, Cheng SH. Molecular genetic mapping of quanti-tative trait loci for milling quality in rice (Oryza sa-tiva L.). J Cereal Sci, 2004, 40(2): 109-114.
[11] 翁建峰, 万向元, 郭涛, 江玲, 翟虎渠, 万建民. 利用CSSL群体研究稻米加工品质相关QTL表达的稳定性. 中国农业科学, 2007, 40(10): 2128-2135.
[12] Wan XY, Wan JM, Weng JF, Jiang L, Bi JC, Wang CM, Zhai HQ. Stability of QTLs for rice grain dimension and endosperm chalkiness characteristics across eight environments. Theor Appl Genet, 2005, 110(7): 1334-1346.
[13] Jiang GH, Hong XY, Xu CG, Li XH, He YQ. Identification of quantitative trait loci for grain appearance and milling quality using a doubled-haploid rice population. J In-tegr Plant Biol, 2005, 47(11): 1391-1403.
[14] Li ZF, Wan JM, Xia JF, Zhai HQ, Ikehashi H. Identifica-tion of quantitative trait loci underlying milling quality of rice (Oryza sativa) grains. Plant Breed, 2004, 123(3): 229-234.
[15] Li ZF, Wan JM, Xia JF, Zhai HQ. Mapping quantitative traits loci underlying appearance quality of rice grains (Oryza sativa L.). Acta Genet Sin, 2003, 30(3): 251-259.
[16] Lou J, Chen L, Yue GH, Lou Qj, Mei HW, Xiong L, Luo LJ. QTL mapping of grain quality traits in rice. J Ce-real Sci, 2009, 50(2): 145-151.
[17] Zheng TQ, Xu JL, Li ZK, Zhai HQ, Wan JM. Genomic regions associated with milling quality and grain shape identified in a set of random introgression lines of rice (Oryza sativa L.). Plant Breed, 2007, 126(2): 158-163.
[18] 梅捍卫, 罗利军, 郭龙彪, 王一平, 余新桥, 应存山, 黎志康. 水稻加工品质数量性状基因座(QTLs)分子定位研究. 遗传学报, 2002, 29(9): 791-797.
[19] 胡霞, 石瑜敏, 贾倩, 徐琴, 王韵, 陈凯, 孙勇, 朱苓华, 徐建龙, 黎志康. 影响水稻穗部性状及籽粒碾磨品质的QTL及其环境互作分析. 作物学报, 2011, 37(7): 1175-1185.
[20] Aluko G, Martinez C, Tohme J, Castano C, Bergman C, Oard JH. QTL mapping of grain quality traits from the interspecific cross Oryza sativa × O. glaberrima. Theor Appl Genet, 2004, 109(3): 630-639.
[21] Li JM, Xiao JH, Grandillo S, Jiang LY, Wan YZ, Deng QY, Yuan LP, McCouch SR. QTL detection for rice grain quality traits using an interspecific backcross population derived from cultivated Asian (O. sativa L.) and Af-rican (O. glaberrima S.) rice. Genome, 2004, 47(4): 697-704.
[22] Septiningsih EM, Trijatmiko KR, Moeljopawiro S, McCouch SR. Identification of quantitative trait loci for grain quality in an advanced backcross population derived from the Oryza sativa variety IR64 and the wild relative O. rufipogon. Theor Appl Genet, 2003, 107(8): 1433-1441.
[23] 刘家富, 奎丽梅, 朱作峰, 谭禄宾, 王桂娟, 黎其万, 束继红, 孙传清. 普通野生稻稻米加工品质和外观品质性状QTL定位. 农业生物技术学报, 2007, 15(1): 90-96.
[24] Mao HL, Sun SY, Yao JL, Wang CR, Yu SB, Xu C
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