综述

细胞抽提物诱导的体细胞重编程

展开
  • 中国农业科学院北京畜牧兽医研究所, 胚胎工程与繁殖技术研究室, 北京 100193

收稿日期: 2012-08-29

  修回日期: 2012-10-09

  网络出版日期: 2013-03-25

基金资助

科技支撑项目(编号:2012BAD12B01)和2013的基本科研业务费项目(编号: 2013ywf-2d-2)资助

Somatic cells reprogramming using cell extracts

Expand
  • Embryo Biotechnology and Reproduction Laboratory, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China

Received date: 2012-08-29

  Revised date: 2012-10-09

  Online published: 2013-03-25

摘要

体细胞重编程是指在特定条件下将已分化的体细胞去分化逆转回原始多能状态或转分化为其他类型细胞。目前诱导体细胞重编程的方法主要有:体细胞核移植、细胞融合、特定转录因子转染、细胞抽提物诱导等。近年来抽提物诱导体细胞重编程越来越受到研究者的关注。利用此法, 通过重编程不仅可以获得需要类型的细胞, 而且方便识别与重编程有关的细胞因子, 探求重编程的机制。文章简略概述诱导体细胞重编程的方法, 并重点阐述细胞抽提物诱导体细胞重编程的研究进展。

本文引用格式

范宗兴,朱化彬,杜卫华 . 细胞抽提物诱导的体细胞重编程[J]. 遗传, 2013 , 35(3) : 262 -268 . DOI: 10.3724/SP.J.1005.2013.00262

Abstract

The process of differentiated somatic cells to a pluripotent state or to another unrelated cell type is referred to as cellular reprogramming. This can be achieved through somatic cell nuclear transfer, cell fusion, specific transcription-factor transduction, and exposure to cell extracts. Cellular reprogramming using cell extracts has gained increasing attention in recent years and has been applied to acquire the target cell types, recognize the functional factors, explore the reprogramming mechanism. In this review, we described different methods of reprogramming somatic cells, which mainly focused on the advances in cellular reprogramming using cell extracts.

参考文献

[1] Wilmut I, Schnieke AE, McWhir J, Kind AJ, Campbell KHS. Viable offspring derived from fetal and adult mammalian cells. Nature, 1997, 385(6619): 810-813.
[2] Miller RA, Ruddle FH. Pluripotent teratocarcinoma-thymus somatic cell hybrids. Cell, 1976, 9(1): 45-55.
[3] Tada M, Tada T, Lefebvre L, Barton SC, Surani MA. Em-bryonic germ cells induce epigenetic reprogramming of somatic nucleus in hybrid cells. EMBO J, 1997, 16(21): 6510-6520.
[4] Tada M, Takahama Y, Abe K, Nakatsuji N, Tada T. Nu-clear reprogramming of somatic cells by in vitro hybridization with ES cells. Curr Biol, 2001, 11(19): 1553-1558.
[5] Takahashi K, Yamanaka S. Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell, 2006, 126(4): 663-676.
[6] Takahashi K, Tanabe K, Ohnuki M, Narita M, Ichisaka T, Tomoda K, Yamanaka S. Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell, 2007, 131(5): 861-872.
[7] 秦彤, 苗向阳. iPS细胞研究的新进展及应用. 遗传, 2010, 32(12): 1205-1214.
[8] Zhao XY, Li W, Lv Z, Liu L, Tong M, Hai T, Hao J, Guo CL, Ma QW, Wang L, Zeng FY, Zhou Q. iPS cells pro-duce viable mice through tetraploid complementation. Nature, 2009, 461(7260): 86-90.
[9] Vierbuchen T, Ostermeier A, Pang ZP, Kokubu Y, Südhof TC, Wernig M. Direct conversion of fibroblasts to functional neurons by defined factors. Nature, 2010, 463(7284): 1035-1041.
[10] Marro S, Pang ZP, Yang N, Tsai MC, Qu K, Chang HY, Südhof TC, Werning M. Direct lineage conversion of terminally differentiated hepatocytes to functional neurons. Cell Stem Cell, 2011, 9(4): 374-382.
[11] Kikyo N, Wade PA, Guschin D, Ge H, Wolffe AP. Active remodeling of somatic nuclei in egg cytoplasm by the nucleosomal ATPase ISWI. Science, 2000, 289(5488): 2360-2362.
[12] Hansis C, Barreto G, Maltry N, Niehrs C. Nuclear repro-gramming of human somatic cells by Xenopus egg extract requires BRG1. Curr Biol, 2004, 14(16): 1475-1480.
[13] Alberio R, Johnson AD, Stick R, Campbell KHS. Differential nuclear remodeling of mammalian somatic cells by Xenopus laevis oocyte and egg cytoplasm. Exp Cell Res, 2005, 307(1): 131-141.
[14] Miyamoto K, Furusawa T, Ohnuki M, Goel S, Tokunaga T, Minami N, Yamada M, Ohsumi K, Imai H. Reprogramming events of mammalian somatic cells induced by Xenopus laevis egg extracts. Mol Reprod Dev, 2007, 74(10): 1268-1277.
[15] Ganier O, Bocquet S, Peiffer I, Brochard V, Arnaud P, Puy A, Jouneau A, Feil R, Renard JP, Méchali M. Synergic re-programming of mammalian cells by combined exposure to mitotic Xenopus egg extracts andtranscription factors. Proc Natl Acad Sci USA, 2011, 108(42): 17331-17336.
[16] Bian YH, Alberio R, Allegrucci C, Campbell KHS, John-son AD. Epigenetic marks in somatic chromatin are remodelled to resemble pluripotent nuclei by amphibian oocyte extracts. Epigenetics, 2009, 4(3): 194-202.
[17] Allegrucci C, Rushton MD, Dixon JE, Sottile V, Shah M, Kumari R, Watson S, Alberio R, Johnson AD. Epigenetic reprogramming of breast cancer cells with oocyte extracts. Mol Cancer, 2011, 10(1): 7.
[18] Bui HT, Wakayama S, Kishigami S, Kim JH, van Thuan N, Wakayama T. The cytoplasm of mouse germinal vesicle stage oocytes can enhance somatic cell nuclear repro-gramming. Development, 2008, 135(23): 3935-3945.
[19] Miyamoto K, Tsukiyama T, Yang Y, Li N, Minami N, Yamada M, Imai H. Cell-free extracts from mammalian oocytes partially induce nuclear reprogramming in somatic cells. Biol Repro, 2009, 80(5): 935-943.
[20] Taranger CK, Noer A, Sørensen AL, Håkelien AM, Bo-quest AC, Collas P. Induction of dedifferentiation, ge-nomewide transcriptional programming, and epigenetic reprogramming by extracts of carcinoma and embryonic-stem cells. Mo
文章导航

/