为揭示猪Opn4基因的结构并研究其在猪视网膜中昼夜表达差异与生长各阶段表达规律, 文章克隆了猪Opn4基因全长CDS区序列, 定量检测了Opn4基因在猪视网膜中昼夜的表达量以及其在不同生长阶段的表达量。结果表明, Opn4基因编码区序列为1 437 bp, 编码478个氨基酸, 分子式为C2398H3705N623O651S23; Opn4基因在白昼的表达量极显著高于夜晚(P<0.01); Opn4基因在出生前34 d胎儿视网膜中表达量最低(P<0.01); 1日龄时表达量最高(P<0.01); 1月龄、4月龄、84月龄猪视网膜中Opn4表达量差异不显著(P>0.05)。以上结果提示, Opn4基因可参与调控猪的生物节律, 并且Opn4主要参与出生后猪对外界光照的应答。
The purpose of this work was to elucidate the characteristics and the expression profiles of porcine Opn4 during developmental stages and different times of the day. The coding sequence of Opn4 gene was cloned. The mRNA expression levels of Opn4 gene in the retina of the swines during developmental stages and different times of the day were examined. The results showed that the swine ORF of Opn4 gene was 1 437 bp in length which encodes 478 amino acids residues with the molecular formula of C2398H3705N623O651S23. The expression level of Opn4 in the day was significantly higher than that in the night (P<0.01). During the growing periods, Opn4 started expressing as early as 34 d in the embryonic phase with the lowest level (P<0.01), reached to a peak 1 day after birth (P<0.01), and then stayed at a moderate standard. The difference of Opn4 expressions among the swines at 1th month, 4th month, and 84th month was not significantly different (P>0.05). In total, results showed that the Opn4 gene can regulate the biological rhythm of the swines. Furthermore, it play an important role in mediate the responsibility to the light in the postnatal swines.
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