蛋白质相互作用实验技术的最新进展
收稿日期: 2013-06-05
修回日期: 2013-08-11
网络出版日期: 2013-10-25
基金资助
国家自然科学基金项目(编号：31171615, 31171543)资助
Recent advances in the techniques of protein-protein interaction study
Received date: 2013-06-05
Revised date: 2013-08-11
Online published: 2013-10-25
王明强 武金霞 张玉红 韩凝 边红武 朱睦元 . 蛋白质相互作用实验技术的最新进展[J]. 遗传, 2013 , 35(11) : 1274 -1283 . DOI: 10.3724/SP.J.1005.2013.01274
Protein-protein interactions play key roles in the development of organisms and the response to biotic and abiotic stresses. Several wet-lab methods have been developed to study this challenging area,including yeast two-hybrid system, tandem affinity purification, Co-immunoprecipitation, GST Pull-down, bimolecular fluorescence complementation, fluorescence resonance energy transfer and surface plasmon resonance analysis. In this review, we discuss theoretical principles and relative advantages and disvantages of these techniques,with an emphasis on recent advances to compensate for limitations.
[1] Hu ZJ. Analysis strategy of protein-protein interaction networks. Methods Mol Biol, 2013, 939: 141–181.<\p>
[2] De Las Rivas J, Fontanillo C. Protein-protein interaction networks: unraveling the wiring of molecular machines within the cell. Brief Funct Genomics, 2012, 11(6): 489– 496.<\p>
[3] Fields S, Song OK. A novel genetic system to detect protein-protein interactions. Nature, 1989, 340(6230): 245– 246.<\p>
[4] Miller JP, Lo RS, Ben-Hur A, Desmarais C, Stagljar I, Noble WS, Fields S. Large-scale identification of yeast integral membrane protein interactions. Proc Natl Acad Sci USA, 2005, 102(34): 12123–12128.<\p>
[5] Natsume T, Nakayama H, Jansson Ö, Isobe T, Takio K, Mikoshiba K. Combination of biomolecular interaction analysis and mass spectrometric amino acid sequencing. Anal Chem, 2000, 72(17): 4193–4198.<\p>
[6] Kanno A, Ozawa T, Umezawa Y. Detection of protein- protein interactions in bacteria by GFP-fragment reconstitution. Methods Mol Biol, 2011, 705: 251–258.<\p>
[7] Suter B, Kittanakom S, Stagljar I. Two-hybrid technologies in proteomics research. Curr Opin Biotechnol, 2008, 19(4): 316–323.<\p>
[8] Luo Y, Batalao A, Zhou H, Zhu L. Mammalian two-hybrid system: a complementary approach to the yeast two-hybrid system. Biotechniques, 1997, 22(2): 350–352.<\p>
[9] Wehr MC, Laage R, Bolz U, Fischer TM, Grünewald S, Scheek S, Bach A, Nave KA, Rossner MJ. Monitoring regulated protein-protein interactions using split TEV. Nat Methods, 2006, 3(12): 985–993.<\p>
[10] Dreze M, Monachello D, Lurin C, Cusick ME, Hill DE, Vidal M, Braun P. High-quality binary interactome mapping. Methods Enzymol, 2010, 470: 281–315.<\p>
[11] Uetz P, Giot L, Cagney G, Mansfield TA, Judson RS, Knight JR, Lockshon D, Narayan V, Srinivasan M, Pochart P, Qureshi-Emili A, Li Y, Godwin B, Conover D, Kalbfleisch T, Vijayadamodar G, Yang MJ, Johnston M, Fields S, Rothberg JM. A comprehensive analysis of protein-protein interactions in Saccharomyces cerevisiae. Nature, 2000, 403(6770): 623–627.<\p>
[12] Ito T, Chiba T, Ozawa R, Yoshida M, Hattori M, Sakaki Y. A comprehensive two-hybrid analysis to explore the yeast protein interactome. Proc Natl Acad Sci USA, 2001, 98(8): 4569–4574.<\p>
[13] Li SM, Armstrong CM. A map of the interactome network of the metazoan C. elegans. Science, 2004, 303(5657): 540–543.<\p>
[14] Giot L, Bader JS, Brouwer C, Chaudhuri A, Kuang B. A protein interaction map of Drosophila melanogaster. Science, 2003, 302(5651): 1727–1736.<\p>
[15] Stelzl U, Worm U, Lalowski M, Haenig C, Brembeck FH, Goehler H, Stroedicke M, Zenkner M, Schoenherr A, Koeppen S, Timm J, Mintzlaff S, Abraham C, Bock N, Kietzmann S, Goedde A, Toksöz E, Droege A, Krobitsch S, Korn B, Birchmeier W, Lehrach H, Wanker EE. A human protein-protein interaction network: a resource for annotating the proteome. Cell, 2005, 122(6): 957–968.<\p>
[16] Singh R, Lee MO, Lee JE, Choi J, Park JH, Kim EH, Yoo RH, Cho JI, Jeon JS, Rakwal R, Agrawal GK, Moon JS, Jwa NS. Rice mitogen-activated protein kinase interactome analysis using the yeast two-hybrid system. Plant Physiol, 2012, 160(1): 477–487.<\p>
[17] Forler D, Köcher T, Rode M, Gentzel M, Izaurralde E, Wilm M. An efficient protein complex purification method for functional proteomics in higher eukaryotes. Nat Biotechnol, 2003, 21(1): 89–92.<\p>
[18] Veraksa A, Bauer A, Artavanis-Tsakonas S. Analyzing protein complexes in Drosophila with tandem affinity purification-mass spectrometry. Dev Dyn, 2005, 232(3): 827–834.<\p>
[19] Bouwmeester T, Bauch A, Ruffner H, Angrand PO, Bergamini G, Croughton K, Cruciat C, Eberhard D, Gagneur J, Ghidelli S, H
/
| 〈 |
|
〉 |
www.chinagene.cn
备案号：京ICP备09063187号-4
总访问:,今日访问:,当前在线:
