基于油桐种子3个不同发育时期转录组的油脂合成代谢途径分析
收稿日期: 2013-06-27
修回日期: 2013-09-07
网络出版日期: 2013-11-25
基金资助
国家林业公益性行业科研专项重大项目(编号：201204403)资助
Analysis of oil synthesis metabolism pathways based on transcrip-tomechanges in tung oil tree’s seeds during three different develop-ment stages
Received date: 2013-06-27
Revised date: 2013-09-07
Online published: 2013-11-25
油桐是我国重要的木本油料植物, 过去对油桐的研究主要集中于栽培和常规育种, 与油桐种仁油脂合成相关的分子机理研究还未见报道。文章采用RNA-seq技术对油桐种子油脂合成的3个不同时期的转录组进行比较, 获得了大量差异表达的Unigene序列。在此基础上, 通过GO分类和Pathway富集性分析将这些差异表达Unigene归类于128个代谢途径, 其中包含与油脂合成相关的脂肪酸生物合成和甘油磷脂代谢途径。桐酸经脂肪酸生物合成途径合成后通过甘油磷脂代谢途径以桐油的形式贮存。将这两个代谢途径的Unigene序列在KEGG数据库中进行比对, 获得了一些关键酶的同源蛋白质。文章通过对编码这些同源蛋白质的基因在油桐种子油脂合成期的表达模式进行分析, 以期为油桐油脂合成, 尤其是桐酸合成机理的解析提供理论参考, 并为油桐的遗传改良提供潜在的基因资源, 从而提高油桐的单位面积产量。
关键词： 油桐(Verniciafordii); 油脂合成途径; RNA-seq; 转录组; 差异表达基因
陈昊 蒋桂雄 龙洪旭 谭晓风 . 基于油桐种子3个不同发育时期转录组的油脂合成代谢途径分析[J]. 遗传, 2013 , 35(12) : 1403 -1414 . DOI: 10.3724/SP.J.1005.2013.01403
Tung oil tree (Vernicia fordii) is one of the important woody oil plants in China. Past researches on tung oil tree mainly focu on the cultivation and conventional breeding while the molecular mechanisms related to tung oil synthesis are still uncovered. We compared transcriptome of tung oil tree’s seeds at three different oil synthesis stages using RNA-seq technology and then obtained a lot of differentially expressed Unigenes. Through GO classification and pathway enrichment analysis, all of these differentially expressed Unigenes were classified into 128 metabolism pathways including fatty acid biosynthesis and glycerophospholipid metabolism which are involved in oil synthesis. Some homologous proteins of key enzymes were obtained when the sequences of the Unigenes within these two pathways were aligned against KEGG data-base. Through analysis of expression profiles of these key enzyme genes during seed’s oil synthesis stage, this research not only shed light on elucidation of plant oil synthesis but also provides candidate genes for genetic improvement of tung oil tree thereby increasing the yield per unit area of tung oil tree.
[1] 谭晓风, 蒋桂雄, 谭方友, 周伟国, 吕平会, 罗克明, 孙汉洲, 王承南, 马锦林, 何佳林, 梁文汇, 黄艳. 我国油桐产业化发展战略调查研究报告. 经济林研究, 2011, 29(3): 1–7. <\p>
[2] 刘金龙, 郑小江, 郑威, 田国政, 杨驰. 油桐品种五爪桐含油量及桐油质量研究. 湖北农业科学, 2011, 50(10): 2031–2035. <\p>
[3] Rock CO, Cronan JE. Escherichia coli as a model for the regulation of dissociable (typeⅡ) fatty acid biosynthesis. Biochim Biophys Acta, 1996, 1302(1): 1–16. <\p>
[4] Ohlrogge J, Browse J. Lipid biosynthesis. Plant Cell, 1995, 7(7): 957–970. <\p>
[5] Shanklin J, Cahoon EB. Desaturation and related modifi-cations of fatty acids. Annu Rev Plant Physiol Plant Mol Biol, 1998, 49: 611–641. <\p>
[6] Nikolau BJ, Ohlrogge JB, Wurtele ES. Plant biotin-containing carboxylases. Arch Biochem Biophys, 2003, 414(2): 211–222. <\p>
[7] Konishi T, Shinohara K, Yamada K, Sasaki Y. Acetyl-CoA carboxylase in higher plants: most plants other than gramineae have both the prokaryotic and the eukaryotic forms of this enzyme. Plant Cell Physiol, 1996, 37(2): 117–122. <\p>
[8] Ruch FE, Vagelos PR. The isolation and general properties of Escherichia coli malonyl coenzyme A-acyl carrier pro-tein transacylase. J Biol Chem, 1973, 248(23): 8086–8094. <\p>
[9] White SW, Zheng J, Zhang YM, Rock CO. The structural biology of type Ⅱ fatty acid biosynthesis. Annu Rev Bio-chem, 2005, 74(1): 791–831. <\p>
[10] Fisher M, Kroon JTM, Martindale W, Stuitje AR, Slabas AR, Rafferty JB. The X-ray structure of Brassica napus β-keto acyl carrier protein reductase and its implications for substrate binding and catalysis. Structure, 2000, 8(4): 339–347. <\p>
[11] Kimber MS, Martin F, Lu Y, Houston S, Vedadi M, Dharamsi A, Fiebig KM, Schmid M, Rock CO. The struc-ture of (3R)-hydroxyacyl-acyl carrier protein dehydratase (FabZ) from Pseudomonas aeruginosa. J Biol Chem, 2004, 279(50): 52593–52602. <\p>
[12] Heath RJ, Rock CO. Enoyl-acyl carrier protein reductase (fabI) plays a determinant role in completing cycles of fatty acid elongation in Escherichia coli. J Biol Chem, 1995, 270(44): 26538–26542. <\p>
[13] Voelker T. Plant acyl-ACP thioesterases: chain-length de-termining enzymes in plant fatty acid biosynthesis. Genet Eng, 1996, 18: 111–133. <\p>
[14] Yuan L, Voelker TA, Hawkins DJ. Modification of the substrate specificity of an acyl-acyl carrier protein thio-esterase by protein engineering. Proc Natl Acad Sci USA, 1995, 92(23): 10639–10643. <\p>
[15] 周奕华, 陈正华. 植物种子中脂肪酸代谢途径的遗传调控与基因工程. 植物学通报, 1998, 15(5): 16–23. <\p>
[16] Jako C, Kumar A, Wei YD, Zou JT, Barton DL, Giblin EM, Covello PS, Taylor DC. Seed-specific over-expression of an Arabidopsis cDNA encoding a diacylglycerol acyl-transferase enhances seed oil content and seed weight. Plant Physiol, 2001, 126(2): 861–874. <\p>
[17] Shockey JM, Gidda SK, Chapital DC, Kuan JC, Dhanoa PK, Bland JM, Rothstein SJ, Mullen RT, Dyer JM. Tung tree DGAT1 and DGAT2 have nonredundant functions in triacylglycerol biosynthesis and are localized to different subdomains of the endoplasmic reticulum. Plant Cell, 2006, 18(9): 2294–2313. <\p>
[18] 汪阳东, 李元, 李鹏. 油桐桐酸合成酶基因克隆和植物表达载体构建. 浙江林业科技, 2007, 27(2): 1–5. <\p>
[19] 李建安, 孙颖, 陈鸿鹏, 刘丽娜, 郭文丹. 油桐LEAFY同源基因片段的克隆与分析. 中南林业科技大学学报, 2008, 28(4): 21–26. <\p>
[20] 李元, 汪阳东, 李鹏, 魏建民. 油桐种子FADX基因的克隆和序列分析. 安徽农业科学, 2008, 36(11): 4753–4755. <\p>
[21] 龙洪旭, 谭晓风, 张琳, 谢禄山, 陈洪. 油桐亲环素基因全长cDNA的克隆及序列分析. 中南林业科技大学学报, 2011, 31(12): 111–117. <\p>
[22] Alagna F, D'Agostino N, Torchia L, Servili M, Rao R, Pietrella M, Giuliano G, Chiusano ML, Baldoni L, Perrotta G. Comparative 454 pyrosequencing of transcripts from two olive genotypes during fruit development. BMC Ge-nomics, 2009, 10(1): 399. <\p>
</
| 〈 |
|
〉 |
www.chinagene.cn
备案号：京ICP备09063187号-4
总访问:,今日访问:,当前在线:
