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猪microRNA组学研究进展

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  • 1. 湖南农业大学动物科学技术学院,长沙 410128;
    2. 畜禽遗传改良湖南省重点实验室,长沙 410128;
    3. 中国科学院亚热带农业生态研究所,长沙 410125;
    4. 中国科学院研究生院,北京 100049
冉茂良,博士研究生,专业方向:猪的遗传育种。E-mail: ranmaoliang0903@126.com

收稿日期: 2014-05-04

  网络出版日期: 2014-10-20

基金资助

国家现代农业产业技术体系建设专项资金(CARS-36)资助

Advances in porcine miRNAome

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  • 1. College of Animal Science & Technology, Hunan Agricultural University, Changsha 410128, China;
    2. Hunan Provincial Key Laboratory for Genetic Improvement of Domestic Animal, Changsha 410128, China;
    3. Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China;
    4. University of the Chinese Academy of Sciences, Beijing 100049, China

Received date: 2014-05-04

  Online published: 2014-10-20

摘要

MicroRNA(miRNA)是一类长约22 nt的非编码小RNA,广泛存在于各种生物中,调节生物体生长、发育和凋亡等过程。研究表明,miRNA在猪肌肉、脂肪、生殖系统以及免疫系统等的发育过程中发挥着重要的调控作用。此外,高通量的新一代测序技术在猪miRNA的挖掘和差异表达研究中发挥着巨大的作用。文章综述了高通量的新一代测序技术在挖掘猪miRNA中的应用以及一些miRNA在猪脂肪代谢、肌肉发育、卵母细胞成熟和B、T淋巴细胞发育中的调控作用,旨在为猪miRNA的研究提供参考,为利用miRNA调控和改善猪肉品质、生长性能、繁殖性能以及免疫机能提供理论基础和研究思路。

本文引用格式

冉茂良,陈斌,尹杰,杨岸奇,李智,蒋明 . 猪microRNA组学研究进展[J]. 遗传, 2014 , 36(10) : 974 -984 . DOI: 10.3724/SP.J.1005.2014.0974

Abstract

MicroRNA (miRNA), a class of non-coding RNA (about 22 nt), widely exists in different organisms and plays an important role in growth, development, and apoptosis. Recently, many studies have shown that miRNAs serve as a regulatory mechanism in mediating the development of pig muscle, fat, reproductive and immunity traits. The next-generation of high-throughput sequencing technology plays a critical role on finding new miRNAs and identifying their different expressions in pig. In this review, we summarize the application of the next-generation of high-throughput sequencing technology in the study of pig miRNAs as well as the regulatory roles of several important miRNAs in fat metabolism, muscle development, oocyte maturation and development of B cells and T cells. This review will provide insight into the research on pig miRNAs, and some ideas on how to improve pork quality, growth, reproductive and immunity performance.

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