陆地棉皱缩叶突变体基因wr3的初步定位
收稿日期: 2014-06-04
网络出版日期: 2014-12-20
基金资助
转基因生物新品种培育科技重大专项子课题(编号：2011ZX08005-001)和转基因特色专用棉新品种培育项目(编号：2011ZX08005-005)资助
Mapping of a new wrinkled leaf (wr3) gene in upland cotton
Received date: 2014-06-04
Online published: 2014-12-20
陆地棉皱缩叶突变体是本项目组自主发现的一种自然突变体。前期对其表型性状的观察发现该突变体的农艺性状有别于前人已报道的皱叶突变体;通过陆陆杂交世代群体对其遗传规律的研究表明, 皱缩叶突变性状是受一对完全隐性基因wr3控制的质量性状。文章以皱叶突变自交系L037和海7124为亲本配置组合得到的海陆杂交F2为作图群体, 利用本实验室遴选的平均覆盖棉花26对染色体上的234对SSR引物(每染色体相对等距离分布9对引物), 通过对双亲以及近等基因池的筛选, 共得到12对多态性引物。用这些引物检测F2作图群体每个单株的标记基因型, 经Join Map 4.0分析显示, 位于Chr.21的引物BNL3279与目的基因连锁, 二者间的遗传距离为28.7 cM。随后对Chr.21上的其他引物进一步筛选, 共得到16对与目的基因连锁的标记, 其中与目的基因距离最近的标记NAU3740的遗传距离为4.8 cM, 另一侧标记cgr5428的遗传距离为10.4 cM。由此推定, 皱缩叶突变体基因wr3在棉花第21染色体上。
李峰利, 狄佳春, 赵亮, 陈旭升 . 陆地棉皱缩叶突变体基因wr3的初步定位[J]. 遗传, 2014 , 36(12) : 1256 -1260 . DOI: 10.3724/SP.J.1005.2014.1256
The phenotype of a wrinkled leaf mutant in upland cotton newly found was different from other analogous mutants reported previously. Genetic analysis indicates that the mutation is controlled by a single recessive gene wr3. In this paper, an F2 mapping population derived from the mutation inbred line L037 crossing with Hai7124 (Gossypium babardense) was used. A total of 12 pairs of polymorphic primers were selected from 234 pairs of SSR primers obtained before, which covered 26 pairs of cotton chromosomes at regular intervals. Genetic mapping of the F2 population showed that gene wr3 was linked to the primer BNL3279 on Chr.21 with the distance of 28.7 cM. Further screening of other primers on Chr.21 showed that gene wr3 was flanked by the primers NAU3740 and cgr5428, with genetic distance of 4.8 cM and 10.4 cM, respectively.
[1] Harland SC. On the genetics of crinkled dwarf rogues in Sea Island cotton. West Ind Bull, 1918, 16(1): 82.
[2] Harland SC. The genetics of cotton. Part IX. Further ex-perience on the inheritance of the crinkled dwarf mutant of G. barbadense L. in interspecific cross and their bearing on Fisher theory of dominance. J Genet, 1933, 28(2): 315-325.
[3] Hutchinson JB, Ghose RU. On the occurrence of crinkled dwarf in Gossypium hirsutum L. J Genet, 1937, 34(3): 437-446.
[4] Turcotte EL, Carl V, Feaster. Inheritance of rugate leaf in pima cotton. J Hered, 1965, 56(5): 234-236.
[5] Kohel RJ. Genetic analysis of the crumpled mutant in Gossypium hirsutum L. Crop Sci, 1973, 13(3): 384-386.
[6] Dilday RH, Kohel RJ, Richmond TR. Genetic analysis of leaf differentiation mutants in upland cotton. Crop Sci, 1975, 15(3): 393-397.
[7] Turcotte EL, Feaster CV. Inheritance of a leaf mutant in American Pima cotton. J Hered, 1980, 71(2): 134-135.
[8] Turcotte EL. Inheritance of a second wrinkled leaf mutant in American Pima cotton. Crop Sci, 1987, 27(4): 702-704.
[9] Kohel RJ. Genetic analysis of the Rex mutant in cotton. J Hered, 1989, 80(1): 78-80.
[10] 简桂良, 马存, 郑传临. 新疆发现皱叶海岛棉. 中国棉花, 1994, 21(2): 20.
[11] 戴日春. 陆地棉新黄绿苗突变体浙12-12N的遗传鉴定. 浙江农业学报, 1995, 7(2): 105-110.
[12] 陈旭升, 狄佳春, 马晓杰. 陆地棉皱缩叶突变性状质量遗传规律分析. 江西农业学报, 2012, 24(4): 7-9.
[13] Paterson AH, Brubaker C, Wendel JF. A rapid method for extraction of cotton (Gossypium spp.) genomic DNA suit-able for RFLP or PCR analysis. Plant Mol Bio Rep, 1993, 11(2): 122-127.
[14] 张天真, 袁有禄, 郭旺珍, Yu J, Kohel RJ. 棉花高强纤维QTLs的微卫星标记筛选.中国农业科学, 2001, 34(4): 363-366. [15] 张军, 郭旺珍, 张天真. 棉花微卫星标记的PAGE/银染快速检测. 棉花学报, 2000, 12(5): 267-269.
[16] Zhang J, Guo WZ, Zhang TZ. Molecular linkage map of allotetraploid cotton (Gossypium hirsutum L. × Gossypi-um barbadense L.) with a haploid population. Theor Appl Genet, 2002, 105: 1166-1174. [17] Van Ooijen, 2006. Joinmap 4, Software for the calculation of genetic linkage maps in experimental populations. Kyazma BV, Wageningen, Netherands. http://www.kyazma.nl/index. php/mc.JoinMap/.
[18] Zhao Liang, Lv Yuanda, Cai Caiping, Tong Xiangchao, Chen Xiangdong, Zhang Wei, Du Hao, Guo Xiuhua, Guo Wangzhen. Toward allotetraploid cotton genome assembly: integration of a high-density molecular genetic linkage map with DNA sequence information. BMC Genomics, 2012, 13: 539-570.
[19] Alonso JM, Stepanova AN, Leisse TJ, Kim CJ, Chen HM, Shinn P, Stevenson DK, Zimmerman J, Barajas P, Cheuk R. Genome-wide insertional mutagenesis of Arabidopsis tha-liana. Science, 2003, 301(5633): 653-657.
[20] 杨松涛, 张涛, 郑家奎. 水稻突变体库研究进展. 中国农学通报, 2010, 26(19): 27-3
[21] 赵亮, 蔡彩平, 张天真, 郭旺珍. 陆地棉红株基因(R1)的精细定位. 科学通报, 2009, 54(7): 888-891.
[22] 宋振云, 杨志敏, 陈旭升. 陆地棉亚红株突变体基因的初步定位. 作物学报, 2007, 33(3): 511-513.
[23] 景超, 马晓杰, 狄佳春, 陈旭升. 陆地棉超矮杆突变体基因的初步定位. 遗传, 2011, 33(12): 1393-1397.
[24] 潘家驹. 棉花育种学. 北京: 中国农业出版社, 1998, 70-72.
/
| 〈 |
|
〉 |
www.chinagene.cn
备案号：京ICP备09063187号-4
总访问:,今日访问:,当前在线:
