遗传图谱表现形式简洁明了,为分析遗传规律、克隆基因提供了便利。Gbrowse、MapViewer等工具虽然能够协助研究人员绘制相似形式的物理图谱,但有很大的局限性:(1)数据需提前布置好;(2)输出结果无法灵活修改。鉴于此,文章基于Perl和SVG语言,开发了一款生物辅助作图软件MapGene2Chrom的本地版与网页版,该软件能够依据输入数据快速绘制相应的物理图谱。该软件输入数据格式简单,输出结果易于修改,图片格式为SVG矢量图,具有很好的移植性,以期为研究人员绘制物理图谱提供便利。
Genetic linkage map is helpful for analysis on heredity of some gene families and map-based gene cloning because of its simple and elegant manifestation. One software is in need to draw a gene physical map, which shows a manner similar to the genetic linkage map, based on the relative physical distance between genes. Although some tools like GBrowse and MapViewer etc. are available to draw gene physical map, there are obvious limitations for them: (1) the data need to be decorated in advance; (2) users can’t modify results. Therefore, we developed a bio-assisted mapping software——MapGene2Chrom with PC and web versions, which is based on Perl and SVG languages. The software can be used to draw the corresponding physical map quickly in SVG format based on the input data. It will become a useful tool for drawing gene physical map with the advantages of simple input data format, easily modified output and very good portability.
[1] Lander ES, Green P, Abrahamson J, Barlow A, Daly MJ, Lincoln SE, Newburg L. MAPMAKER: an interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics , 1987, 1(2): 174-181.
[2] VAN Ooijen JW. Multipoint maximum likelihood mapping in a full-sib family of an outbreeding species. Genet Res , 2011, 93(5): 343-349.
[3] Stam P. Construction of integrated genetic linkage maps by means of a new computer package: Join Map. Plant J , 1993, 3(5): 739-744.
[4] Stam P. JoinMap 2. 0 deals with all types of plant mapping populations. In: Plant Genome III Abstracts. San Diego, USA, 1995.
[5] 刘仁虎, 孟金陵. MapDraw, 在Excel中绘制遗传连锁图的宏. 遗传, 2003, 25(3): 317-321.
[6] 沈利爽, 郑先武, 朱立煌. Mapplotter——个输出遗传图谱、图示基因型和QTL曲线图形的软件. 遗传, 2000, 22(3): 172-174.
[7] Stein LD, Mungall C, Shu SQ, Caudy M, Mangone M, Day A, Nickerson E, Stajich JE, Harris TW, Arva A, Lewis S. The generic genome browser: a building block for a model organism system database. Genome Res , 2002, 12(10): 1599-1610.